Alteration of the tumor suppressor gene p53 in a high fraction of hormone refractory prostate cancer.

Journal Article (Journal Article;Review)

PURPOSE: We studied the role of p53 tumor suppressor gene alteration in prostate cancer progression by demonstrating a difference in abnormal p53 findings between early and hormone refractory disease. MATERIALS AND METHODS: The study included p53 immunohistochemistry of 26 archival transurethral resection specimens from patients with radiation recurrent and hormone refractory disease, 27 untreated primary tumors and 8 untreated metastatic lesions. p53 mutation analysis of tumor deoxyribonucleic acid (DNA) from microdissected specimens was done by cold single strand conformational polymorphism and DNA sequencing. RESULTS: Elevated p53 protein was present in 16 of 17 hormone refractory specimens (94%), 4 of 8 untreated metastatic tumors (50%) and 6 of 27 primary untreated tumors (22%). DNA analysis of representative specimens with elevated p53 confirmed p53 gene alterations in 9 of 11 cases (82%). CONCLUSIONS: Our study revealed a clear progression of increased p53 alteration from untreated primary to hormone refractory disease (p < 0.00005).

Full Text

Duke Authors

Cited Authors

  • Heidenberg, HB; Sesterhenn, IA; Gaddipati, JP; Weghorst, CM; Buzard, GS; Moul, JW; Srivastava, S

Published Date

  • August 1995

Published In

Volume / Issue

  • 154 / 2 Pt 1

Start / End Page

  • 414 - 421

PubMed ID

  • 7609105

International Standard Serial Number (ISSN)

  • 0022-5347

Digital Object Identifier (DOI)

  • 10.1097/00005392-199508000-00024


  • eng

Conference Location

  • United States