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Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines.

Publication ,  Journal Article
Nakamura, K; Yasunaga, Y; Segawa, T; Ko, D; Moul, JW; Srivastava, S; Rhim, JS
Published in: Int J Oncol
October 2002

Curcumin, traditionally used as a seasoning spice in Indian cuisine, has been reported to decrease the proliferation potential of prostate cancer cells, by a mechanism that is not fully understood. In the current study, we have evaluated the effects of curcumin in cell growth, activation of signal transduction, and transforming activities of both androgen-dependent and independent cell lines. Prostate cancer cell lines, LNCaP and PC-3, were treated with curcumin and its effects were further analyzed on signal transduction and expression of androgen receptor (AR) and AR-related cofactors using transient transfection assay and Western blotting. Our results show that curcumin down-regulates transactivation and expression of AR, activator protein-1 (AP-1), nuclear factor-kappaB (NF-kappaB), and CREB (cAMP response element-binding protein)-binding protein (CBP). Curcumin also inhibited the transforming activities of both cell lines as evidenced by the reduced colony forming ability in soft agar. The results obtained here demonstrate that curcumin has a potential therapeutic effect on prostate cancer cells through down-regulation of AR and AR-related cofactors (AP-1, NF-kappaB and CBP).

Duke Scholars

Published In

Int J Oncol

ISSN

1019-6439

Publication Date

October 2002

Volume

21

Issue

4

Start / End Page

825 / 830

Location

Greece

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription Factor AP-1
  • Signal Transduction
  • Receptors, Androgen
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • NF-kappa B
  • Male
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nakamura, K., Yasunaga, Y., Segawa, T., Ko, D., Moul, J. W., Srivastava, S., & Rhim, J. S. (2002). Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. Int J Oncol, 21(4), 825–830.
Nakamura, Keiichiro, Yutaka Yasunaga, Takehiko Segawa, Daejin Ko, Judd W. Moul, Shiv Srivastava, and Johng S. Rhim. “Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines.Int J Oncol 21, no. 4 (October 2002): 825–30.
Nakamura K, Yasunaga Y, Segawa T, Ko D, Moul JW, Srivastava S, et al. Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. Int J Oncol. 2002 Oct;21(4):825–30.
Nakamura, Keiichiro, et al. “Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines.Int J Oncol, vol. 21, no. 4, Oct. 2002, pp. 825–30.
Nakamura K, Yasunaga Y, Segawa T, Ko D, Moul JW, Srivastava S, Rhim JS. Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. Int J Oncol. 2002 Oct;21(4):825–830.

Published In

Int J Oncol

ISSN

1019-6439

Publication Date

October 2002

Volume

21

Issue

4

Start / End Page

825 / 830

Location

Greece

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription Factor AP-1
  • Signal Transduction
  • Receptors, Androgen
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • NF-kappa B
  • Male
  • Humans