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p53 regulates the expression of the tumor suppressor gene maspin.

Publication ,  Journal Article
Zou, Z; Gao, C; Nagaich, AK; Connell, T; Saito, S; Moul, JW; Seth, P; Appella, E; Srivastava, S
Published in: J Biol Chem
March 3, 2000

Maspin has been shown to inhibit tumor cell invasion and metastasis in breast tumor cells. Maspin expression was detected in normal breast and prostate epithelial cells, whereas tumor cells exhibited reduced or no expression. However, the regulatory mechanism of maspin expression remains unknown. We report here a rapid and robust induction of maspin expression in prostate cancer cells (LNCaP, DU145, and PC3) and breast tumor cells (MCF7) following wild type p53 expression from an adenovirus p53 expression vector (AdWTp53). p53 activates the maspin promoter by binding directly to the p53 consensus-binding site present in the maspin promoter. DNA-damaging agents and cytotoxic drugs induced endogenous maspin expression in cells containing the wild type p53. Maspin expression was refractory to the DNA-damaging agents in cells containing mutant p53. These results, combined with recent studies of the tumor metastasis suppressor gene KAI1 and plasminogen activator inhibitor 1 (PAI1), define a new category of molecular targets of p53 that have the potential to negatively regulate tumor invasion and/or metastasis.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 3, 2000

Volume

275

Issue

9

Start / End Page

6051 / 6054

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Serpins
  • RNA, Messenger
  • Proteins
  • Prostatic Neoplasms
  • Promoter Regions, Genetic
  • Neoplasm Metastasis
  • Male
 

Citation

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Zou, Z., Gao, C., Nagaich, A. K., Connell, T., Saito, S., Moul, J. W., … Srivastava, S. (2000). p53 regulates the expression of the tumor suppressor gene maspin. J Biol Chem, 275(9), 6051–6054. https://doi.org/10.1074/jbc.275.9.6051
Zou, Z., C. Gao, A. K. Nagaich, T. Connell, S. Saito, J. W. Moul, P. Seth, E. Appella, and S. Srivastava. “p53 regulates the expression of the tumor suppressor gene maspin.J Biol Chem 275, no. 9 (March 3, 2000): 6051–54. https://doi.org/10.1074/jbc.275.9.6051.
Zou Z, Gao C, Nagaich AK, Connell T, Saito S, Moul JW, et al. p53 regulates the expression of the tumor suppressor gene maspin. J Biol Chem. 2000 Mar 3;275(9):6051–4.
Zou, Z., et al. “p53 regulates the expression of the tumor suppressor gene maspin.J Biol Chem, vol. 275, no. 9, Mar. 2000, pp. 6051–54. Pubmed, doi:10.1074/jbc.275.9.6051.
Zou Z, Gao C, Nagaich AK, Connell T, Saito S, Moul JW, Seth P, Appella E, Srivastava S. p53 regulates the expression of the tumor suppressor gene maspin. J Biol Chem. 2000 Mar 3;275(9):6051–6054.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 3, 2000

Volume

275

Issue

9

Start / End Page

6051 / 6054

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Serpins
  • RNA, Messenger
  • Proteins
  • Prostatic Neoplasms
  • Promoter Regions, Genetic
  • Neoplasm Metastasis
  • Male