Skip to main content

Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model.

Publication ,  Journal Article
Li, Z; Rakkar, A; Katayose, Y; Kim, M; Shanmugam, N; Srivastava, S; Moul, JW; McLeod, DG; Cowan, KH; Seth, P
Published in: Gene Ther
May 1998

Infection of Renca cells in vitro with a recombinant adenovirus expressing a marker gene beta-galactosidase resulted in high level of the transgene expression. Renca tumors grown in Balb/C mice were also infectable with this recombinant adenovirus. The transgene expression in the tumors lasted for about 7 days, however, administration of another dose of Ad-beta gal, on day 7 produced beta-galactosidase expression. To investigate the effect of antibodies to adenovirus, animals were injected with multiple doses of adenovirus to produce neutralizing antibodies. To these animals Renca cells were injected and tumors formed. Interestingly, when Ad beta-gal was administered into these tumors, a high level of transgene expression was still observed. We next explored the utility of a recombinant adenovirus expressing p53 (AdWTp53) in the Renca tumor model. Renca cells when exposed to an adenovirus expressing p53 (AdWTp53) produced a high level of p53 protein, a p53-inducible gene p21/WAF1/Cip1 and underwent apoptosis. A single injection of AdWTp53 (10(9) plaque forming units) resulted in significant inhibition of tumor growth. However, multiple administrations (four doses of 2.5 x 10(8) plaque forming units) of AdWTp53 were needed for tumor cures. Mixing uninfected and AdWTp53-infected cells showed a bystander effect of AdWTp53-infected Renca cells. Based on these results we believe that an appropriate dose scheduling of AdWTp53 can be efficacious for cancer gene therapy in immune-competent tumor-bearing animals.

Duke Scholars

Published In

Gene Ther

DOI

ISSN

0969-7128

Publication Date

May 1998

Volume

5

Issue

5

Start / End Page

605 / 613

Location

England

Related Subject Headings

  • beta-Galactosidase
  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Neoplasm Transplantation
  • Mice, Inbred BALB C
  • Mice
  • Kidney Neoplasms
  • Immunocompetence
  • Genetic Vectors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, Z., Rakkar, A., Katayose, Y., Kim, M., Shanmugam, N., Srivastava, S., … Seth, P. (1998). Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model. Gene Ther, 5(5), 605–613. https://doi.org/10.1038/sj.gt.3300636
Li, Z., A. Rakkar, Y. Katayose, M. Kim, N. Shanmugam, S. Srivastava, J. W. Moul, D. G. McLeod, K. H. Cowan, and P. Seth. “Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model.Gene Ther 5, no. 5 (May 1998): 605–13. https://doi.org/10.1038/sj.gt.3300636.
Li Z, Rakkar A, Katayose Y, Kim M, Shanmugam N, Srivastava S, et al. Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model. Gene Ther. 1998 May;5(5):605–13.
Li, Z., et al. “Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model.Gene Ther, vol. 5, no. 5, May 1998, pp. 605–13. Pubmed, doi:10.1038/sj.gt.3300636.
Li Z, Rakkar A, Katayose Y, Kim M, Shanmugam N, Srivastava S, Moul JW, McLeod DG, Cowan KH, Seth P. Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model. Gene Ther. 1998 May;5(5):605–613.

Published In

Gene Ther

DOI

ISSN

0969-7128

Publication Date

May 1998

Volume

5

Issue

5

Start / End Page

605 / 613

Location

England

Related Subject Headings

  • beta-Galactosidase
  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Neoplasm Transplantation
  • Mice, Inbred BALB C
  • Mice
  • Kidney Neoplasms
  • Immunocompetence
  • Genetic Vectors