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Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation.

Publication ,  Journal Article
Srivastava, S; Katayose, D; Tong, YA; Craig, CR; McLeod, DG; Moul, JW; Cowan, KH; Seth, P
Published in: Urology
December 1995

OBJECTIVES: A recombinant adenovirus vector (AdWTp53) expressing wild-type p53 was evaluated for its cell growth inhibitory effects on metastatic human prostate cancer cells. METHODS: Human prostate cancer cells LNCaP, DU145, PC3, 1LN, and DUPro-1 were infected with AdWTp53 vector and expression of exogenous p53 in these cells was analyzed by immunoprecipitation and western blot assays. The cell growth inhibitory effects of AdWTp53 were determined by counting cell number on a hemocytometer or by crystal violet staining of cells after infection with AdWTp53. The p53-regulated gene WAF1 and DNA fragmentation were also analyzed in prostate cancer cells infected with AdWTp53. RESULTS: High levels of the AdWTp53 vector-derived p53 protein were present in metastatic prostate cancer cells, and the p53-regulated gene WAF1 was induced in these cells. Infection of these tumor cell lines with AdWTp53 vector resulted in severe growth inhibition and cell death in comparison to untreated or control adenovirus vector-infected cells. Furthermore, fragmentation of genomic DNA, a property associated with apoptosis, was also observed in prostate cancer cells infected with AdWTp53. CONCLUSIONS: AdWTp53 vector exhibited a potent inhibitory effect on the growth of all of human metastatic prostate cancer cells, and both cytostatic and cytotoxic effects of AdWTp53 were observed. The induction of p53-regulated gene WAF1 in AdWTp53-infected prostate cancer cells suggests the involvement of cellular p53 pathway in the cell growth inhibition. These results provide a molecular basis for further evaluation of antitumorigenic effects of AdWTp53 vector in animal models of prostate cancer.

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Published In

Urology

DOI

ISSN

0090-4295

Publication Date

December 1995

Volume

46

Issue

6

Start / End Page

843 / 848

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Recombination, Genetic
  • Prostatic Neoplasms
  • Precipitin Tests
  • Male
  • Humans
  • Genetic Vectors
  • DNA, Neoplasm
 

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Srivastava, S., Katayose, D., Tong, Y. A., Craig, C. R., McLeod, D. G., Moul, J. W., … Seth, P. (1995). Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation. Urology, 46(6), 843–848. https://doi.org/10.1016/S0090-4295(99)80355-0
Srivastava, S., D. Katayose, Y. A. Tong, C. R. Craig, D. G. McLeod, J. W. Moul, K. H. Cowan, and P. Seth. “Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation.Urology 46, no. 6 (December 1995): 843–48. https://doi.org/10.1016/S0090-4295(99)80355-0.
Srivastava S, Katayose D, Tong YA, Craig CR, McLeod DG, Moul JW, et al. Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation. Urology. 1995 Dec;46(6):843–8.
Srivastava, S., et al. “Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation.Urology, vol. 46, no. 6, Dec. 1995, pp. 843–48. Pubmed, doi:10.1016/S0090-4295(99)80355-0.
Srivastava S, Katayose D, Tong YA, Craig CR, McLeod DG, Moul JW, Cowan KH, Seth P. Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation. Urology. 1995 Dec;46(6):843–848.
Journal cover image

Published In

Urology

DOI

ISSN

0090-4295

Publication Date

December 1995

Volume

46

Issue

6

Start / End Page

843 / 848

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Recombination, Genetic
  • Prostatic Neoplasms
  • Precipitin Tests
  • Male
  • Humans
  • Genetic Vectors
  • DNA, Neoplasm