Skip to main content

A novel human cancer culture model for the study of prostate cancer.

Publication ,  Journal Article
Yasunaga, Y; Nakamura, K; Ko, D; Srivastava, S; Moul, JW; Sesterhenn, IA; McLeod, DG; Rhim, JS
Published in: Oncogene
November 29, 2001

Research into molecular and genetic mechanisms underlying prostate carcinogenesis would be greatly advanced by in vitro models of prostate tumors representing primary tumors. We have successfully established an immortalized human prostate epithelial (HPE) cell culture derived from a primary tumor with telomerase. The actively proliferating early passaged RC-58T cells were transduced through infection with a retrovirus vector expressing the human telomerase catalytic subunit (hTERT). A high level of telomerase was detected in RC-58T/hTERT cells but not RC-58T cells. RC-58T/hTERT cells are currently growing well at passage 50, whereas RC-58T cells senesced at passage 7. RC-58T/hTERT cells exhibit transformed morphology. More importantly, these immortalized cells showed anchorage-independent growth as they formed colonies in soft agar and grew above the agar layer. Expression of androgen-regulated prostate specific gene NKX3.1 and epithelial specific cytokeratin 8 (CK8) but not prostate specific antigen (PSA) and androgen receptor was detected in RC-58T/hTERT cells. Prostate stem cell antigen (PSCA) and p16 were also expressed in this cell line. RC-58T/hTERT cells showed growth inhibition when exposed to retinoic acid and transforming growth factor (TGF)-beta1 known potent inhibitors of prostate epithelial cell growth. A number of chromosome alterations were observed including the loss of chromosomes Y, 3p, 10p, 17p, 18q and the gain of chromosomes 16 and 20. These results demonstrate that this primary tumor-derived HPE cell line retained its transformed phenotypes and should allow studies to elucidate molecular and genetic alterations involved in prostate cancer. This is the first documented case of an established human prostate cancer cell line from a primary tumor of a prostate cancer patient with telomerase.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

November 29, 2001

Volume

20

Issue

55

Start / End Page

8036 / 8041

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tretinoin
  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Transduction, Genetic
  • Telomerase
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retroviridae
  • RNA, Neoplasm
  • Prostatic Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yasunaga, Y., Nakamura, K., Ko, D., Srivastava, S., Moul, J. W., Sesterhenn, I. A., … Rhim, J. S. (2001). A novel human cancer culture model for the study of prostate cancer. Oncogene, 20(55), 8036–8041. https://doi.org/10.1038/sj.onc.1205002
Yasunaga, Y., K. Nakamura, D. Ko, S. Srivastava, J. W. Moul, I. A. Sesterhenn, D. G. McLeod, and J. S. Rhim. “A novel human cancer culture model for the study of prostate cancer.Oncogene 20, no. 55 (November 29, 2001): 8036–41. https://doi.org/10.1038/sj.onc.1205002.
Yasunaga Y, Nakamura K, Ko D, Srivastava S, Moul JW, Sesterhenn IA, et al. A novel human cancer culture model for the study of prostate cancer. Oncogene. 2001 Nov 29;20(55):8036–41.
Yasunaga, Y., et al. “A novel human cancer culture model for the study of prostate cancer.Oncogene, vol. 20, no. 55, Nov. 2001, pp. 8036–41. Pubmed, doi:10.1038/sj.onc.1205002.
Yasunaga Y, Nakamura K, Ko D, Srivastava S, Moul JW, Sesterhenn IA, McLeod DG, Rhim JS. A novel human cancer culture model for the study of prostate cancer. Oncogene. 2001 Nov 29;20(55):8036–8041.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

November 29, 2001

Volume

20

Issue

55

Start / End Page

8036 / 8041

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Tretinoin
  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Transduction, Genetic
  • Telomerase
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retroviridae
  • RNA, Neoplasm
  • Prostatic Neoplasms