An essential role for a MEK-C/EBP pathway during growth factor-regulated cortical neurogenesis.

Published

Journal Article

Mammalian neurogenesis is determined by an interplay between intrinsic genetic mechanisms and extrinsic cues such as growth factors. Here we have defined a signaling cascade, a MEK-C/EBP pathway, that is essential for cortical progenitor cells to become postmitotic neurons. Inhibition of MEK or of the C/EBP family of transcription factors inhibits neurogenesis while expression of a C/EBPbeta mutant that is a phosphorylation-mimic at a MEK-Rsk site enhances neurogenesis. C/EBP mediates this positive effect by direct transcriptional activation of neuron-specific genes such as Talpha1 alpha-tubulin. Conversely, inhibition of C/EBP-dependent transcription enhances CNTF-mediated generation of astrocytes from the same progenitor cells. Thus, activation of a MEK-C/EBP pathway enhances neurogenesis and inhibits gliogenesis, thereby providing a mechanism whereby growth factors can selectively bias progenitors to become neurons during development.

Full Text

Cited Authors

  • Ménard, C; Hein, P; Paquin, A; Savelson, A; Yang, XM; Lederfein, D; Barnabé-Heider, F; Mir, AA; Sterneck, E; Peterson, AC; Johnson, PF; Vinson, C; Miller, FD

Published Date

  • November 2002

Published In

Volume / Issue

  • 36 / 4

Start / End Page

  • 597 - 610

PubMed ID

  • 12441050

Pubmed Central ID

  • 12441050

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/s0896-6273(02)01026-7

Language

  • eng