Interferon regulatory factors are transcriptional regulators of adipogenesis.

Published

Journal Article

We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.

Full Text

Duke Authors

Cited Authors

  • Eguchi, J; Yan, Q-W; Schones, DE; Kamal, M; Hsu, C-H; Zhang, MQ; Crawford, GE; Rosen, ED

Published Date

  • January 2008

Published In

Volume / Issue

  • 7 / 1

Start / End Page

  • 86 - 94

PubMed ID

  • 18177728

Pubmed Central ID

  • 18177728

International Standard Serial Number (ISSN)

  • 1550-4131

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2007.11.002

Language

  • eng

Conference Location

  • United States