Dynamics of the epigenetic landscape during erythroid differentiation after GATA1 restoration.

Published

Journal Article

Interplays among lineage-specific nuclear proteins, chromatin modifying enzymes, and the basal transcription machinery govern cellular differentiation, but their dynamics of action and coordination with transcriptional control are not fully understood. Alterations in chromatin structure appear to establish a permissive state for gene activation at some loci, but they play an integral role in activation at other loci. To determine the predominant roles of chromatin states and factor occupancy in directing gene regulation during differentiation, we mapped chromatin accessibility, histone modifications, and nuclear factor occupancy genome-wide during mouse erythroid differentiation dependent on the master regulatory transcription factor GATA1. Notably, despite extensive changes in gene expression, the chromatin state profiles (proportions of a gene in a chromatin state dominated by activating or repressive histone modifications) and accessibility remain largely unchanged during GATA1-induced erythroid differentiation. In contrast, gene induction and repression are strongly associated with changes in patterns of transcription factor occupancy. Our results indicate that during erythroid differentiation, the broad features of chromatin states are established at the stage of lineage commitment, largely independently of GATA1. These determine permissiveness for expression, with subsequent induction or repression mediated by distinctive combinations of transcription factors.

Full Text

Duke Authors

Cited Authors

  • Wu, W; Cheng, Y; Keller, CA; Ernst, J; Kumar, SA; Mishra, T; Morrissey, C; Dorman, CM; Chen, K-B; Drautz, D; Giardine, B; Shibata, Y; Song, L; Pimkin, M; Crawford, GE; Furey, TS; Kellis, M; Miller, W; Taylor, J; Schuster, SC; Zhang, Y; Chiaromonte, F; Blobel, GA; Weiss, MJ; Hardison, RC

Published Date

  • October 2011

Published In

Volume / Issue

  • 21 / 10

Start / End Page

  • 1659 - 1671

PubMed ID

  • 21795386

Pubmed Central ID

  • 21795386

Electronic International Standard Serial Number (EISSN)

  • 1549-5469

Digital Object Identifier (DOI)

  • 10.1101/gr.125088.111

Language

  • eng

Conference Location

  • United States