Development of a liquid chromatography-tandem mass spectrometry assay of six antimicrobials in plasma for pharmacokinetic studies in premature infants.


Journal Article

This method provides a simple extraction procedure, as well as a validated, sensitive, and specific liquid chromatography-tandem mass spectrometry assay for the simultaneous quantification of ampicillin, piperacillin, tazobactam, meropenem, acyclovir, and metronidazole in human plasma. The method was validated over concentration ranges specific for each compound, with a lower limit of quantification of 50-300 ng/mL and a sample volume of 50 μL. The method is accurate and precise, with within- and between-day accuracy ranging from 85 to 110% and 92 to 110%, respectively, and within- and between-day precision of 89-111% and 91-109%, respectively. Simplicity, low plasma volume, and high throughput make this method suitable for clinical pharmacokinetic studies in premature infants.

Full Text

Duke Authors

Cited Authors

  • Cohen-Wolkowiez, M; White, NR; Bridges, A; Benjamin, DK; Kashuba, ADM

Published Date

  • November 2011

Published In

Volume / Issue

  • 879 / 30

Start / End Page

  • 3497 - 3506

PubMed ID

  • 21983195

Pubmed Central ID

  • 21983195

Electronic International Standard Serial Number (EISSN)

  • 1873-376X

International Standard Serial Number (ISSN)

  • 1570-0232

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2011.09.031


  • eng