In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.
Embryonal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.
Duke Scholars
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- Zebrafish Proteins
- Zebrafish
- Tumor Cells, Cultured
- Time Factors
- Rhabdomyosarcoma, Embryonal
- Recombinant Fusion Proteins
- Oncology & Carcinogenesis
- Neovascularization, Pathologic
- Neoplasm Transplantation
- Neoplasm Invasiveness
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Zebrafish Proteins
- Zebrafish
- Tumor Cells, Cultured
- Time Factors
- Rhabdomyosarcoma, Embryonal
- Recombinant Fusion Proteins
- Oncology & Carcinogenesis
- Neovascularization, Pathologic
- Neoplasm Transplantation
- Neoplasm Invasiveness