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In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.

Publication ,  Journal Article
Ignatius, MS; Chen, E; Elpek, NM; Fuller, AZ; Tenente, IM; Clagg, R; Liu, S; Blackburn, JS; Linardic, CM; Rosenberg, AE; Nielsen, PG ...
Published in: Cancer Cell
May 15, 2012

Embryonal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.

Duke Scholars

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Published In

Cancer Cell

DOI

EISSN

1878-3686

Publication Date

May 15, 2012

Volume

21

Issue

5

Start / End Page

680 / 693

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Tumor Cells, Cultured
  • Time Factors
  • Rhabdomyosarcoma, Embryonal
  • Recombinant Fusion Proteins
  • Oncology & Carcinogenesis
  • Neovascularization, Pathologic
  • Neoplasm Transplantation
  • Neoplasm Invasiveness
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ignatius, M. S., Chen, E., Elpek, N. M., Fuller, A. Z., Tenente, I. M., Clagg, R., … Langenau, D. M. (2012). In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma. Cancer Cell, 21(5), 680–693. https://doi.org/10.1016/j.ccr.2012.03.043
Ignatius, Myron S., Eleanor Chen, Natalie M. Elpek, Adam Z. Fuller, Inês M. Tenente, Ryan Clagg, Sali Liu, et al. “In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.Cancer Cell 21, no. 5 (May 15, 2012): 680–93. https://doi.org/10.1016/j.ccr.2012.03.043.
Ignatius MS, Chen E, Elpek NM, Fuller AZ, Tenente IM, Clagg R, et al. In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma. Cancer Cell. 2012 May 15;21(5):680–93.
Ignatius, Myron S., et al. “In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.Cancer Cell, vol. 21, no. 5, May 2012, pp. 680–93. Pubmed, doi:10.1016/j.ccr.2012.03.043.
Ignatius MS, Chen E, Elpek NM, Fuller AZ, Tenente IM, Clagg R, Liu S, Blackburn JS, Linardic CM, Rosenberg AE, Nielsen PG, Mempel TR, Langenau DM. In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma. Cancer Cell. 2012 May 15;21(5):680–693.
Journal cover image

Published In

Cancer Cell

DOI

EISSN

1878-3686

Publication Date

May 15, 2012

Volume

21

Issue

5

Start / End Page

680 / 693

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Tumor Cells, Cultured
  • Time Factors
  • Rhabdomyosarcoma, Embryonal
  • Recombinant Fusion Proteins
  • Oncology & Carcinogenesis
  • Neovascularization, Pathologic
  • Neoplasm Transplantation
  • Neoplasm Invasiveness