Urinary biomarkers of oxidative status in a clinical model of oxidative assault.
Journal Article (Journal Article)
BACKGROUND: We used doxorubicin-based chemotherapy as a clinical model of oxidative assault in humans. METHODS: The study recruited newly diagnosed breast cancer patients (n = 23). Urine samples were collected immediately before (T0) and at 1 hour (T1) and 24 hours (T24) after i.v. administration of treatment. Measurements included allantoin and the isoprostanes iPF(2alpha)-III, iPF(2alpha)-VI, and 8,12-iso-iPF(2alpha)-VI along with the prostaglandin 2,3-dinor-iPF(2alpha)-III, a metabolite of iPF(2alpha)-III. All biomarkers were quantified using liquid chromatography-tandem mass spectrometry. RESULTS: In all subjects, the levels of the biomarkers increased at T1: allantoin by 22% (P = 0.06), iPF(2alpha)-III by 62% (P < 0.05), iPF(2alpha)-VI by 41% (P < 0.05), 8,12-iso-iPF(2alpha)-VI by 58% (P < 0.05), and 2,3-dinor-iPF(2alpha)-III by 52% (P < 0.05). At T24, the F2-isoprostanes returned to their baseline levels; the levels of allantoin continued to increase, although the T24-T0 difference was not statistically significant. CONCLUSIONS: These results indicate that urinary F2-isoprostanes are valid biomarkers and allantoin is a promising biomarker of oxidative status in humans. IMPACT: The levels of biomarkers change quickly in response to oxidative assault and can be used to monitor oxidative status in humans in response to treatments related either to generation of free radicals (chemotherapy and radiation therapy) or to antioxidants (inborn metabolic diseases and Down syndrome).
Full Text
Duke Authors
- Di Giulio, Richard T.
- Ilyasova, Dora
- Marcom, Paul Kelly
- Marks, Jeffrey R.
- Millington, David Stuart
- Spasojevic, Ivan
- Young, Sarah Phyllis
Cited Authors
- Il'yasova, D; Spasojevic, I; Wang, F; Tolun, AA; Base, K; Young, SP; Marcom, PK; Marks, J; Mixon, G; DiGiulio, R; Millington, DS
Published Date
- June 2010
Published In
Volume / Issue
- 19 / 6
Start / End Page
- 1506 - 1510
PubMed ID
- 20501773
Pubmed Central ID
- PMC2883001
Electronic International Standard Serial Number (EISSN)
- 1538-7755
Digital Object Identifier (DOI)
- 10.1158/1055-9965.EPI-10-0211
Language
- eng
Conference Location
- United States