Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.

Journal Article (Journal Article)

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.

Full Text

Duke Authors

Cited Authors

  • Tolun, AA; Zhang, H; Il'yasova, D; Sztáray, J; Young, SP; Millington, DS

Published Date

  • July 15, 2010

Published In

Volume / Issue

  • 402 / 2

Start / End Page

  • 191 - 193

PubMed ID

  • 20361921

Pubmed Central ID

  • PMC3595192

Electronic International Standard Serial Number (EISSN)

  • 1096-0309

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2010.03.033

Language

  • eng

Conference Location

  • United States