Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
Published
Journal Article
Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.
Full Text
Duke Authors
Cited Authors
- Tolun, AA; Zhang, H; Il'yasova, D; Sztáray, J; Young, SP; Millington, DS
Published Date
- July 15, 2010
Published In
Volume / Issue
- 402 / 2
Start / End Page
- 191 - 193
PubMed ID
- 20361921
Pubmed Central ID
- 20361921
Electronic International Standard Serial Number (EISSN)
- 1096-0309
Digital Object Identifier (DOI)
- 10.1016/j.ab.2010.03.033
Language
- eng
Conference Location
- United States