Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.
Publication
, Journal Article
Zhang, H; Il'yasova, D; Sztaray, J; Young, SP; Wang, F; Millington, DS
Published in: Anal Biochem
April 15, 2010
F(2)-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F(2alpha), a urinary metabolite of 8-iso-prostaglandin F(2alpha.) Urine was purified by solid-phase extraction and analyzed by UPLC-MS/MS with negative-ion electrospray ionization. The method was robust with a mean inaccuracy of 9%, interday and intraday imprecision of 7.5% or lower, and a lower limit of quantification of 0.5 microg/L, equivalent to 0.04 pmol injected onto the column. An analysis time of 6 min was shorter than previously published methods and amenable to large studies.
Duke Scholars
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
April 15, 2010
Volume
399
Issue
2
Start / End Page
302 / 304
Location
United States
Related Subject Headings
- Tandem Mass Spectrometry
- Oxidative Stress
- Humans
- F2-Isoprostanes
- Chromatography, High Pressure Liquid
- Biomarkers
- Biochemistry & Molecular Biology
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Zhang, H., Il’yasova, D., Sztaray, J., Young, S. P., Wang, F., & Millington, D. S. (2010). Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry. Anal Biochem, 399(2), 302–304. https://doi.org/10.1016/j.ab.2009.12.024
Zhang, Haoyue, Dora Il’yasova, Judit Sztaray, Sarah P. Young, Frances Wang, and David S. Millington. “Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.” Anal Biochem 399, no. 2 (April 15, 2010): 302–4. https://doi.org/10.1016/j.ab.2009.12.024.
Zhang H, Il’yasova D, Sztaray J, Young SP, Wang F, Millington DS. Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry. Anal Biochem. 2010 Apr 15;399(2):302–4.
Zhang, Haoyue, et al. “Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.” Anal Biochem, vol. 399, no. 2, Apr. 2010, pp. 302–04. Pubmed, doi:10.1016/j.ab.2009.12.024.
Zhang H, Il’yasova D, Sztaray J, Young SP, Wang F, Millington DS. Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry. Anal Biochem. 2010 Apr 15;399(2):302–304.
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
April 15, 2010
Volume
399
Issue
2
Start / End Page
302 / 304
Location
United States
Related Subject Headings
- Tandem Mass Spectrometry
- Oxidative Stress
- Humans
- F2-Isoprostanes
- Chromatography, High Pressure Liquid
- Biomarkers
- Biochemistry & Molecular Biology
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology