Phagocytic cells synthesize 19-nor-10-keto-25-hydroxyvitamin D3, a metabolite that may induce differentiation of the human monoblastic cell line U937.

Published

Journal Article

Phagocytic cells, including normal human blood neutrophils and monocytes, metabolize 25-hydroxyvitamin D3 in vitro to more polar metabolites. Cells of the human monoblastic cell line U937 produced three metabolites when incubated with 25-hydroxyvitamin D3. One of these metabolites, previously designated peak III, has its maximal absorbance at 310 nm. Mass spectral analysis of the trimethylsilylated derivatives of peak III revealed a spectral pattern very similar to that published for the trimethylsilylated derivatives of 19-nor-10-keto-25-hydroxyvitamin D3. The biosynthetic 19-nor-10-keto-25-hydroxyvitamin D3 was active in the induction of differentiation of U937 cells.

Full Text

Duke Authors

Cited Authors

  • Gray, TK; Millington, DS; Maltby, DA; Williams, ME; Cohen, MS; Dodd, RC

Published Date

  • December 1985

Published In

Volume / Issue

  • 82 / 23

Start / End Page

  • 8218 - 8221

PubMed ID

  • 3865223

Pubmed Central ID

  • 3865223

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.82.23.8218

Language

  • eng

Conference Location

  • United States