Combined high-performance liquid chromatographic-continuous-flow fast atom bombardment mass spectrometric analysis of acylcoenzyme A compounds.
A high-performance liquid chromatographic method for the analysis of coenzyme A thioesters which employs continuous-flow fast atom bombardment mass spectrometric detection is presented. The chromatographic system utilizes gradient elution with reversed-phase conditions using ammonium acetate-acetonitrile from both standard analytical (3.9 mm I.D.) and microbore (1 mm I.D.) columns. Applications to coenzyme A thioesters of various acyl group chain length (C2-C18) and functionality (-COOH, -OH, -C = C-) are described. The system is also applied to an in vitro enzyme reaction (crotonase) to directly follow the disappearance of substrate and appearance of product. The mass spectrometry of coenzyme A thioesters, their chromatographic behavior, system stability, and sensitivity of detection are discussed.
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- Mass Spectrometry
- Enoyl-CoA Hydratase
- Chromatography, High Pressure Liquid
- Acyl Coenzyme A
Citation
Published In
DOI
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Mass Spectrometry
- Enoyl-CoA Hydratase
- Chromatography, High Pressure Liquid
- Acyl Coenzyme A