Biomedical applications of high-performance liquid chromatography-mass spectrometry with continuous-flow fast atom bombardment.

Journal Article (Journal Article)

This report describes the application of high-performance liquid chromatography combined with continuous-flow fast atom bombardment mass spectrometry to analytical problems in the biomedical laboratory. Applications include the compound-specific detection of diagnostic acylcarnitines in human urine, the separation and analysis of acyl-coenzyme A thioesters, and qualitative studies on complex mixtures of modified peptides (dansyl and dinitrophenyl derivatives). For each of these applications standard analytical columns (3.9 mm I.D.) and 1 ml/min flow-rates were employed with post-column stream splitting (1:100) before mass spectrometry. Various mobile phase compositions and solvent gradients were employed. The addition of 1-5% glycerol to the mobile phase was shown to have little effect on the chromatography. For all compounds studied (acylcarnitines, acyl-coenzyme A thioesters, and derivatized peptides) molecular weight information was obtained and sufficient sensitivity was achieved to allow unambiguous identification of trace components in complex mixtures.

Full Text

Duke Authors

Cited Authors

  • Millington, DS; Norwood, DL; Kodo, N; Moore, R; Green, MD; Berman, J

Published Date

  • January 2, 1991

Published In

  • J Chromatogr

Volume / Issue

  • 562 / 1-2

Start / End Page

  • 47 - 58

PubMed ID

  • 2026712

Digital Object Identifier (DOI)

  • 10.1016/0378-4347(91)80563-r


  • eng

Conference Location

  • Netherlands