The STEDMAN project: biophysical, biochemical and metabolic effects of a behavioral weight loss intervention during weight loss, maintenance, and regain.

Journal Article (Journal Article)

The Study of the Effects of Diet on Metabolism and Nutrition (STEDMAN) Project uses comprehensive metabolic profiling to probe biochemical mechanisms of weight loss in humans. Measurements at baseline, 2 and 4 weeks, 6 and 12 months included diet, body composition, metabolic rate, hormones, and 80 intermediary metabolites measured by mass spectrometry. In 27 obese adults in a behavioral weight loss intervention, median weight decreased 13.9 lb over the first 6 months, then reverted towards baseline by 12 months. Insulin resistance (HOMA) was partially ameliorated in the first 6 months and showed sustained improvement at 12 months despite weight regain. Ghrelin increased with weight loss and reverted to baseline, whereas leptin and PYY fell at 6 months and remained persistently low. NPY levels did not change. Factors possibly contributing to sustained improvement in insulin sensitivity despite weight regain include adiponectin (increased by 12 months), IGF-1 (increased during weight loss and continued to increase during weight regain), and visceral fat (fell at 6 months but did not change thereafter). We observed a persistent reduction in free fatty acids, branched chain amino acids, and related metabolites that may contribute to improved insulin action. These findings provide evidence for sustained benefits of weight loss in obese humans and insights into mechanisms.

Full Text

Duke Authors

Cited Authors

  • Lien, LF; Haqq, AM; Arlotto, M; Slentz, CA; Muehlbauer, MJ; McMahon, RL; Rochon, J; Gallup, D; Bain, JR; Ilkayeva, O; Wenner, BR; Stevens, RD; Millington, DS; Muoio, DM; Butler, MD; Newgard, CB; Svetkey, LP

Published Date

  • February 2009

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 21 - 35

PubMed ID

  • 19290809

Pubmed Central ID

  • PMC2678563

Electronic International Standard Serial Number (EISSN)

  • 1557-8100

Digital Object Identifier (DOI)

  • 10.1089/omi.2008.0035


  • eng

Conference Location

  • United States