Advances in targeted genome editing.

Published

Journal Article (Review)

New technologies have recently emerged that enable targeted editing of genomes in diverse systems. This includes precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. This progress has been facilitated by advances in engineering targeted nucleases with programmable, site-specific DNA-binding domains, including zinc finger proteins and transcription activator-like effectors (TALEs). Recent improvements have enhanced nuclease performance, accelerated nuclease assembly, and lowered the cost of genome editing. These advances are driving new approaches to many areas of biotechnology, including biopharmaceutical production, agriculture, creation of transgenic organisms and cell lines, and studies of genome structure, regulation, and function. Genome editing is also being investigated in preclinical and clinical gene therapies for many diseases.

Full Text

Duke Authors

Cited Authors

  • Perez-Pinera, P; Ousterout, DG; Gersbach, CA

Published Date

  • August 2012

Published In

Volume / Issue

  • 16 / 3-4

Start / End Page

  • 268 - 277

PubMed ID

  • 22819644

Pubmed Central ID

  • 22819644

Electronic International Standard Serial Number (EISSN)

  • 1879-0402

International Standard Serial Number (ISSN)

  • 1367-5931

Digital Object Identifier (DOI)

  • 10.1016/j.cbpa.2012.06.007

Language

  • eng