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A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs.

Publication ,  Journal Article
Eward, WC; Mito, JK; Eward, CA; Carter, JE; Ferrer, JM; Kirsch, DG; Brigman, BE
Published in: Clin Orthop Relat Res
March 2013

BACKGROUND: Treatment of soft tissue sarcoma (STS) includes complete tumor excision. However, in some patients, residual sarcoma cells remain in the tumor bed. We previously described a novel hand-held imaging device prototype that uses molecular imaging to detect microscopic residual cancer in mice during surgery. QUESTIONS/PURPOSES: To test this device in a clinical trial of dogs with naturally occurring sarcomas, we asked: (1) Are any adverse clinical or laboratory effects observed after intravenous administration of the fluorescent probes? (2) Do canine sarcomas exhibit fluorescence after administration of the cathepsin-activated probe? (3) Is the tumor-to-background ratio sufficient to distinguish tumor from tumor bed? And (4) can residual fluorescence be detected in the tumor bed during surgery and does this correlate with a positive margin? METHODS: We studied nine dogs undergoing treatment for 10 STS or mast cell tumors. Dogs received an intravenous injection of VM249, a fluorescent probe that becomes optically active in the presence of cathepsin proteases. After injection, tumors were removed by wide resection. The tumor bed was imaged using the novel imaging device to search for residual fluorescence. We determined correlations between tissue fluorescence and histopathology, cathepsin protease expression, and development of recurrent disease. Minimum followup was 9 months (mean, 12 months; range, 9-15 months). RESULTS: Fluorescence was apparent from all 10 tumors and ranged from 3 × 10(7) to 1 × 10(9) counts/millisecond/cm(2). During intraoperative imaging, normal skeletal muscle showed no residual fluorescence. Histopathologic assessment of surgical margins correlated with intraoperative imaging in nine of 10 cases; in the other case, there was no residual fluorescence, but tumor was found at the margin on histologic examination. No animals had recurrent disease at 9 to 15 months. CONCLUSIONS: These initial findings suggest this imaging system might be useful to intraoperatively detect residual tumor after wide resections. CLINICAL RELEVANCE: The ability to assess the tumor bed intraoperatively for residual disease has the potential to improve local control.

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Published In

Clin Orthop Relat Res

DOI

EISSN

1528-1132

Publication Date

March 2013

Volume

471

Issue

3

Start / End Page

834 / 842

Location

United States

Related Subject Headings

  • Time Factors
  • Soft Tissue Neoplasms
  • Sarcoma
  • Orthopedics
  • Neoplasm, Residual
  • Neoplasm Recurrence, Local
  • Molecular Imaging
  • Male
  • Injections, Intravenous
  • Fluorescent Dyes
 

Citation

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ICMJE
MLA
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Eward, W. C., Mito, J. K., Eward, C. A., Carter, J. E., Ferrer, J. M., Kirsch, D. G., & Brigman, B. E. (2013). A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs. Clin Orthop Relat Res, 471(3), 834–842. https://doi.org/10.1007/s11999-012-2560-8
Eward, William C., Jeffrey K. Mito, Cindy A. Eward, Jessica E. Carter, Jorge M. Ferrer, David G. Kirsch, and Brian E. Brigman. “A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs.Clin Orthop Relat Res 471, no. 3 (March 2013): 834–42. https://doi.org/10.1007/s11999-012-2560-8.
Eward WC, Mito JK, Eward CA, Carter JE, Ferrer JM, Kirsch DG, et al. A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs. Clin Orthop Relat Res. 2013 Mar;471(3):834–42.
Eward, William C., et al. “A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs.Clin Orthop Relat Res, vol. 471, no. 3, Mar. 2013, pp. 834–42. Pubmed, doi:10.1007/s11999-012-2560-8.
Eward WC, Mito JK, Eward CA, Carter JE, Ferrer JM, Kirsch DG, Brigman BE. A novel imaging system permits real-time in vivo tumor bed assessment after resection of naturally occurring sarcomas in dogs. Clin Orthop Relat Res. 2013 Mar;471(3):834–842.
Journal cover image

Published In

Clin Orthop Relat Res

DOI

EISSN

1528-1132

Publication Date

March 2013

Volume

471

Issue

3

Start / End Page

834 / 842

Location

United States

Related Subject Headings

  • Time Factors
  • Soft Tissue Neoplasms
  • Sarcoma
  • Orthopedics
  • Neoplasm, Residual
  • Neoplasm Recurrence, Local
  • Molecular Imaging
  • Male
  • Injections, Intravenous
  • Fluorescent Dyes