Discriminating between latent and active tuberculosis with multiple biomarker responses.
Published
Journal Article
We sought to identify biomarker responses to tuberculosis specific antigens which could 1) improve the diagnosis of tuberculosis infection and 2) allow the differentiation of active and latent infections. Seventy subjects with active tuberculosis (N = 12), latent tuberculosis (N = 32), or no evidence of tuberculosis infection (N = 26) were evaluated. We used the Luminex Multiplexed Bead Array platform to simultaneously evaluate 25 biomarkers in the supernatant of whole blood samples following overnight stimulation using the Quantiferon(®) Gold In-Tube kit. We defined the response to stimulation as the difference (within an individual patient) between the response to the pooled tuberculosis antigens and the negative control. IP-10 response was significantly higher in tuberculosis-infected (active or latent) subjects compared to the uninfected group (p < 0.0001). Among the 25 parameters, expression levels of IL-15 and MCP-1 were found to be significantly higher in the active tuberculosis group compared to the latent tuberculosis group (p = 0.0006 and 0.0030, respectively). When combined, IL-15 and MCP-1 accurately identified 83% of active and 88% of latent infections. The combination of IL-15 and MCP-1 responses was accurate in distinguishing persons with active tuberculosis from persons with latent tuberculosis in this study.
Full Text
Duke Authors
Cited Authors
- Frahm, M; Goswami, ND; Owzar, K; Hecker, E; Mosher, A; Cadogan, E; Nahid, P; Ferrari, G; Stout, JE
Published Date
- May 2011
Published In
Volume / Issue
- 91 / 3
Start / End Page
- 250 - 256
PubMed ID
- 21393062
Pubmed Central ID
- 21393062
Electronic International Standard Serial Number (EISSN)
- 1873-281X
Digital Object Identifier (DOI)
- 10.1016/j.tube.2011.02.006
Language
- eng
Conference Location
- Scotland