Skip to main content
Journal cover image

Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism.

Publication ,  Journal Article
Lewis, DA; Stashenko, GJ; Akay, OM; Price, LI; Owzar, K; Ginsburg, GS; Chi, J-T; Ortel, TL
Published in: Thromb Res
December 2011

INTRODUCTION: Venous thromboembolism may recur in up to 30% of patients with a spontaneous venous thromboembolism after a standard course of anticoagulation. Identification of patients at risk for recurrent venous thromboembolism would facilitate decisions concerning the duration of anticoagulant therapy. OBJECTIVES: In this exploratory study, we investigated whether whole blood gene expression data could distinguish subjects with single venous thromboembolism from subjects with recurrent venous thromboembolism. METHODS: 40 adults with venous thromboembolism (23 with single event and 17 with recurrent events) on warfarin were recruited. Individuals with antiphospholipid syndrome or cancer were excluded. Plasma and serum samples were collected for biomarker testing, and PAXgene tubes were used to collect whole blood RNA samples. RESULTS: D-dimer levels were significantly higher in patients with recurrent venous thromboembolism, but P-selectin and thrombin-antithrombin complex levels were similar in the two groups. Comparison of gene expression data from the two groups provided us with a 50 gene probe model that distinguished these two groups with good receiver operating curve characteristics (AUC 0.75). This model includes genes involved in mRNA splicing and platelet aggregation. Pathway analysis between subjects with single and recurrent venous thromboembolism revealed that the Akt pathway was up-regulated in the recurrent venous thromboembolism group compared to the single venous thromboembolism group. CONCLUSIONS: In this exploratory study, gene expression profiles of whole blood appear to be a useful strategy to distinguish subjects with single venous thromboembolism from those with recurrent venous thromboembolism. Prospective studies with additional patients are needed to validate these results.

Duke Scholars

Published In

Thromb Res

DOI

EISSN

1879-2472

Publication Date

December 2011

Volume

128

Issue

6

Start / End Page

536 / 540

Location

United States

Related Subject Headings

  • Venous Thrombosis
  • Up-Regulation
  • Risk Factors
  • Recurrence
  • Peptide Hydrolases
  • P-Selectin
  • Middle Aged
  • Male
  • Humans
  • Genomics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lewis, D. A., Stashenko, G. J., Akay, O. M., Price, L. I., Owzar, K., Ginsburg, G. S., … Ortel, T. L. (2011). Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism. Thromb Res, 128(6), 536–540. https://doi.org/10.1016/j.thromres.2011.06.003
Lewis, Deborah A., Gregg J. Stashenko, Olga M. Akay, Lulit I. Price, Kouros Owzar, Geoffrey S. Ginsburg, Jen-Tsan Chi, and Thomas L. Ortel. “Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism.Thromb Res 128, no. 6 (December 2011): 536–40. https://doi.org/10.1016/j.thromres.2011.06.003.
Lewis DA, Stashenko GJ, Akay OM, Price LI, Owzar K, Ginsburg GS, et al. Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism. Thromb Res. 2011 Dec;128(6):536–40.
Lewis, Deborah A., et al. “Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism.Thromb Res, vol. 128, no. 6, Dec. 2011, pp. 536–40. Pubmed, doi:10.1016/j.thromres.2011.06.003.
Lewis DA, Stashenko GJ, Akay OM, Price LI, Owzar K, Ginsburg GS, Chi J-T, Ortel TL. Whole blood gene expression analyses in patients with single versus recurrent venous thromboembolism. Thromb Res. 2011 Dec;128(6):536–540.
Journal cover image

Published In

Thromb Res

DOI

EISSN

1879-2472

Publication Date

December 2011

Volume

128

Issue

6

Start / End Page

536 / 540

Location

United States

Related Subject Headings

  • Venous Thrombosis
  • Up-Regulation
  • Risk Factors
  • Recurrence
  • Peptide Hydrolases
  • P-Selectin
  • Middle Aged
  • Male
  • Humans
  • Genomics