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Mice lacking the dopamine transporter display altered regulation of distal colonic motility.

Publication ,  Journal Article
Walker, JK; Gainetdinov, RR; Mangel, AW; Caron, MG; Shetzline, MA
Published in: Am J Physiol Gastrointest Liver Physiol
August 2000

The mechanisms by which dopamine (DA) influences gastrointestinal (GI) tract motility are incompletely understood and complicated by tissue- and species-specific differences in dopaminergic function. To improve the understanding of DA action on GI motility, we used an organ tissue bath system to characterize motor function of distal colonic smooth muscle segments from wild-type and DA transporter knockout (DAT -/-) mice. In wild-type mice, combined blockade of D(1) and D(2) receptors resulted in significant increases in tone (62 +/- 9%), amplitude of spontaneous phasic contractions (167 +/- 24%), and electric field stimulation (EFS)-induced (40 +/- 8%) contractions, suggesting that endogenous DA is inhibitory to mouse distal colonic motility. The amplitudes of spontaneous phasic and EFS-induced contractions were lower in DAT -/- mice relative to wild-type mice. These differences were eliminated by combined D(1) and D(2) receptor blockade, indicating that the inhibitory effects of DA on distal colonic motility are potentiated in DAT -/- mice. Motility index was decreased but spontaneous phasic contraction frequency was enhanced in DAT -/- mice relative to wild-type mice. The fact that spontaneous phasic and EFS-induced contractile activity were altered by the lack of the DA transporter suggests an important role for endogenous DA in modulating motility of mouse distal colon.

Duke Scholars

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

August 2000

Volume

279

Issue

2

Start / End Page

G311 / G318

Location

United States

Related Subject Headings

  • Sulpiride
  • Stimulation, Chemical
  • Receptors, Dopamine D1
  • Potassium Chloride
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Transport Proteins
  • Membrane Glycoproteins
 

Citation

APA
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MLA
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Walker, J. K., Gainetdinov, R. R., Mangel, A. W., Caron, M. G., & Shetzline, M. A. (2000). Mice lacking the dopamine transporter display altered regulation of distal colonic motility. Am J Physiol Gastrointest Liver Physiol, 279(2), G311–G318. https://doi.org/10.1152/ajpgi.2000.279.2.G311
Walker, J. K., R. R. Gainetdinov, A. W. Mangel, M. G. Caron, and M. A. Shetzline. “Mice lacking the dopamine transporter display altered regulation of distal colonic motility.Am J Physiol Gastrointest Liver Physiol 279, no. 2 (August 2000): G311–18. https://doi.org/10.1152/ajpgi.2000.279.2.G311.
Walker JK, Gainetdinov RR, Mangel AW, Caron MG, Shetzline MA. Mice lacking the dopamine transporter display altered regulation of distal colonic motility. Am J Physiol Gastrointest Liver Physiol. 2000 Aug;279(2):G311–8.
Walker, J. K., et al. “Mice lacking the dopamine transporter display altered regulation of distal colonic motility.Am J Physiol Gastrointest Liver Physiol, vol. 279, no. 2, Aug. 2000, pp. G311–18. Pubmed, doi:10.1152/ajpgi.2000.279.2.G311.
Walker JK, Gainetdinov RR, Mangel AW, Caron MG, Shetzline MA. Mice lacking the dopamine transporter display altered regulation of distal colonic motility. Am J Physiol Gastrointest Liver Physiol. 2000 Aug;279(2):G311–G318.

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

August 2000

Volume

279

Issue

2

Start / End Page

G311 / G318

Location

United States

Related Subject Headings

  • Sulpiride
  • Stimulation, Chemical
  • Receptors, Dopamine D1
  • Potassium Chloride
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Transport Proteins
  • Membrane Glycoproteins