Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1-infected donor: implications for vaccine design.
Plasma from a small subset of subjects chronically infected with HIV-1 shows remarkable magnitude and breadth of neutralizing activity. From one of these individuals (CH0219), we isolated two broadly neutralizing antibodies (bnAbs), CH01 and VRC-CH31, from two clonal lineages of memory B cells with distinct specificities (variable loop 1 and 2 [V1V2] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of CH0219 serum neutralization breadth. These data provide proof of concept for an HIV-1 vaccine that aims to elicit bnAbs of multiple specificities.
Bonsignori, M; Montefiori, DC; Wu, X; Chen, X; Hwang, K-K; Tsao, C-Y; Kozink, DM; Parks, RJ; Tomaras, GD; Crump, JA; Kapiga, SH; Sam, NE; Kwong, PD; Kepler, TB; Liao, H-X; Mascola, JR; Haynes, BF
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