Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41.
A component to the problem of inducing broad neutralizing HIV-1 gp41 membrane proximal external region (MPER) antibodies is the need to focus the antibody response to the transiently exposed MPER pre-hairpin intermediate neutralization epitope. Here we describe a HIV-1 envelope (Env) gp140 oligomer prime followed by MPER peptide-liposomes boost strategy for eliciting serum antibody responses in rhesus macaques that bind to a gp41 fusion intermediate protein. This Env-liposome immunization strategy induced antibodies to the 2F5 neutralizing epitope ⁶⁶⁴DKW residues, and these antibodies preferentially bound to a gp41 fusion intermediate construct as well as to MPER scaffolds stabilized in the 2F5-bound conformation. However, no serum lipid binding activity was observed nor was serum neutralizing activity for HIV-1 pseudoviruses present. Nonetheless, the Env-liposome prime-boost immunization strategy induced antibodies that recognized a gp41 fusion intermediate protein and was successful in focusing the antibody response to the desired epitope.
Dennison, SM; Sutherland, LL; Jaeger, FH; Anasti, KM; Parks, R; Stewart, S; Bowman, C; Xia, S-M; Zhang, R; Shen, X; Scearce, RM; Ofek, G; Yang, Y; Kwong, PD; Santra, S; Liao, H-X; Tomaras, G; Letvin, NL; Chen, B; Alam, SM; Haynes, BF
Volume / Issue
Start / End Page
Pubmed Central ID
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)