Isolation of a human anti-HIV gp41 membrane proximal region neutralizing antibody by antigen-specific single B cell sorting.

Published

Journal Article

Broadly neutralizing antibodies are not commonly produced in HIV-1 infected individuals nor by experimental HIV-1 vaccines. When these antibodies do occur, it is important to be able to isolate and characterize them to provide clues for vaccine design. CAP206 is a South African subtype C HIV-1-infected individual previously shown to have broadly neutralizing plasma antibodies targeting the envelope gp41 distal membrane proximal external region (MPER). We have now used a fluoresceinated peptide tetramer antigen with specific cell sorting to isolate a human neutralizing monoclonal antibody (mAb) against the HIV-1 envelope gp41 MPER. The isolated recombinant mAb, CAP206-CH12, utilized a portion of the distal MPER (HXB2 amino acid residues, 673-680) and neutralized a subset of HIV-1 pseudoviruses sensitive to CAP206 plasma antibodies. Interestingly, this mAb was polyreactive and used the same germ-line variable heavy (V(H)1-69) and variable kappa light chain (V(K)3-20) gene families as the prototype broadly neutralizing anti-MPER mAb, 4E10 (residues 672-680). These data indicate that there are multiple immunogenic targets in the C-terminus of the MPER of HIV-1 gp41 envelope and suggests that gp41 neutralizing epitopes may interact with a restricted set of naive B cells during HIV-1 infection.

Full Text

Duke Authors

Cited Authors

  • Morris, L; Chen, X; Alam, M; Tomaras, G; Zhang, R; Marshall, DJ; Chen, B; Parks, R; Foulger, A; Jaeger, F; Donathan, M; Bilska, M; Gray, ES; Abdool Karim, SS; Kepler, TB; Whitesides, J; Montefiori, D; Moody, MA; Liao, H-X; Haynes, BF

Published Date

  • 2011

Published In

Volume / Issue

  • 6 / 9

Start / End Page

  • e23532 -

PubMed ID

  • 21980336

Pubmed Central ID

  • 21980336

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0023532

Language

  • eng

Conference Location

  • United States