An electronic method for counting human T and B cell rosettes.

Published

Journal Article

This study reports the adaptation of an electronic particle-counting method for the enumeration of T cell and B cell rosettes of human lymphocytes. This method and the conventional hemocytometer method were compared in parallel on seventeen T cell and seven B cell rosette enumerations in a blind study design. All data were analyzed for statistical significance; correlation coefficents were calculated. There was significant agreement between both methods for T cells at p less than 0.001 (mean particle counter 80.6% +/-11.3 mean hemocytometer 76.7+/-8.2%) and for the B cells at p less than 0.002 (mean particle counter 22.5+/-9.7, mean hemocytometer 23.4+/-5.12). These results demonstrate that the electronic particle counter is useful as a rapid, convenient, semi-automated method for the enumeration of T cell and B cell rosettes.

Full Text

Duke Authors

Cited Authors

  • Denny, TN; Kinsley, KM; Benson, JW; Bull, RW

Published Date

  • 1979

Published In

Volume / Issue

  • 37 / 4

Start / End Page

  • 244 - 250

PubMed ID

  • 315130

Pubmed Central ID

  • 315130

International Standard Serial Number (ISSN)

  • 0042-9007

Digital Object Identifier (DOI)

  • 10.1111/j.1423-0410.1979.tb02298.x

Language

  • eng

Conference Location

  • England