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22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats.

Publication ,  Journal Article
Kuriyama, S; Denny, TN; Aviv, A
Published in: J Cardiovasc Pharmacol
June 1988

To gain further insight into differences in cellular Na+ and K+ regulation between the spontaneously hypertensive rat (SHR), Wistar Kyoto (WKY), and American Wistar (W) rats, 22Na+ and 86Rb+ washouts were performed under steady-state conditions in cultured vascular smooth muscle cells from the three rat strains. SHR vascular smooth muscle cells showed significantly higher bumetanide sensitive 86Rb+ washout rate constant (x 10(-4)/min; mean +/- SEM) than WKY cells (-38.6 +/- 2.84 and -23.8 +/- 3.58, respectively; p less than 0.005). SHR vascular smooth muscle cells also exhibited significantly higher values than WKY cells in the total 22Na+ washout rate constant (x 10(-2)/min) (-61.0 +/- 1.57 vs. -53.8 +/- 1.24; p less than 0.005). The amiloride sensitive component of the 22Na+ washout rate constant accounted for these differences (-18.6 +/- 1.04 for SHR and -12.1 +/- 2.00 for WKY; p less than 0.05). There were no apparent differences in cellular Na+ concentrations between WKY and SHR cells. In general, the 86Rb+ and 22Na+ washout parameters of W rat cells were quite similar to those of cells from SHR. We conclude that the bumetanide-sensitive 86Rb+ washout (the Na+ K+-cotransport), the overall, and the amiloride-sensitive 22Na+ washout (the latter primarily represents the Na+/H+ antiport) are higher in SHR than WKY rat vascular smooth muscle cells. These findings indicate innate differences in cellular Na+ and K+ transport in vascular smooth muscle cells of the SHR and WKY rat. The mechanisms responsible for these differences are yet to be determined.

Duke Scholars

Published In

J Cardiovasc Pharmacol

DOI

ISSN

0160-2446

Publication Date

June 1988

Volume

11

Issue

6

Start / End Page

722 / 729

Location

United States

Related Subject Headings

  • Species Specificity
  • Sodium Radioisotopes
  • Sodium
  • Rubidium Radioisotopes
  • Rubidium
  • Rats, Inbred WKY
  • Rats, Inbred Strains
  • Rats, Inbred SHR
  • Rats
  • Ouabain
 

Citation

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Kuriyama, S., Denny, T. N., & Aviv, A. (1988). 22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats. J Cardiovasc Pharmacol, 11(6), 722–729. https://doi.org/10.1097/00005344-198806000-00014
Kuriyama, S., T. N. Denny, and A. Aviv. “22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats.J Cardiovasc Pharmacol 11, no. 6 (June 1988): 722–29. https://doi.org/10.1097/00005344-198806000-00014.
Kuriyama S, Denny TN, Aviv A. 22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats. J Cardiovasc Pharmacol. 1988 Jun;11(6):722–9.
Kuriyama, S., et al. “22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats.J Cardiovasc Pharmacol, vol. 11, no. 6, June 1988, pp. 722–29. Pubmed, doi:10.1097/00005344-198806000-00014.
Kuriyama S, Denny TN, Aviv A. 22Na+ and 86Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats. J Cardiovasc Pharmacol. 1988 Jun;11(6):722–729.

Published In

J Cardiovasc Pharmacol

DOI

ISSN

0160-2446

Publication Date

June 1988

Volume

11

Issue

6

Start / End Page

722 / 729

Location

United States

Related Subject Headings

  • Species Specificity
  • Sodium Radioisotopes
  • Sodium
  • Rubidium Radioisotopes
  • Rubidium
  • Rats, Inbred WKY
  • Rats, Inbred Strains
  • Rats, Inbred SHR
  • Rats
  • Ouabain