Evaluation of an atypical HIV type 1 antibody. Serologic pattern leading to detection of HIV type 2 infection in North America.

Journal Article (Journal Article)

The variability and discordance of human immunodeficiency virus type 1 (HIV-1) antibody enzyme immunoassay determinations on serial specimens derived, to our knowledge, from the first documented case of HIV-2 infection in North America are described. The initial specimen was weakly reactive, but two subsequent serum specimens were both nonreactive by enzyme immunoassay. All specimens were indeterminate for HIV-1 antibody by HIV-1 Western blot analysis. Serum HIV-2 antibody was demonstrated by enzyme immunoassay using whole virus lysate, HIV-2-specific synthetic peptide assays, and HIV-2 Western blot analysis. Human immunodeficiency virus type 2 genomic sequences were demonstrated in peripheral blood mononuclear cells using gene amplification technology. Human immunodeficiency virus type 2, isolated from peripheral blood lymphocytes, had typical morphologic features of lenti-virus by electron microscopy. Western blot analysis and other specific assays should be considered in individuals with clinical evidence suggesting HIV infection who are nonreactive for HIV-1 antibody by enzyme immunoassay or who have atypical reactivity patterns.

Full Text

Duke Authors

Cited Authors

  • Lombardo, JM; Horsburgh, CR; Denny, TN; Michaels, J; Schable, C; Meyenhofer, MF; Shriver, K; Sharer, LR; Ou, CY; Rayfield, MA

Published Date

  • November 1989

Published In

Volume / Issue

  • 113 / 11

Start / End Page

  • 1245 - 1249

PubMed ID

  • 2684088

International Standard Serial Number (ISSN)

  • 0003-9985


  • eng

Conference Location

  • United States