Evaluation of an atypical HIV type 1 antibody. Serologic pattern leading to detection of HIV type 2 infection in North America.
Journal Article (Journal Article)
The variability and discordance of human immunodeficiency virus type 1 (HIV-1) antibody enzyme immunoassay determinations on serial specimens derived, to our knowledge, from the first documented case of HIV-2 infection in North America are described. The initial specimen was weakly reactive, but two subsequent serum specimens were both nonreactive by enzyme immunoassay. All specimens were indeterminate for HIV-1 antibody by HIV-1 Western blot analysis. Serum HIV-2 antibody was demonstrated by enzyme immunoassay using whole virus lysate, HIV-2-specific synthetic peptide assays, and HIV-2 Western blot analysis. Human immunodeficiency virus type 2 genomic sequences were demonstrated in peripheral blood mononuclear cells using gene amplification technology. Human immunodeficiency virus type 2, isolated from peripheral blood lymphocytes, had typical morphologic features of lenti-virus by electron microscopy. Western blot analysis and other specific assays should be considered in individuals with clinical evidence suggesting HIV infection who are nonreactive for HIV-1 antibody by enzyme immunoassay or who have atypical reactivity patterns.
Full Text
Duke Authors
Cited Authors
- Lombardo, JM; Horsburgh, CR; Denny, TN; Michaels, J; Schable, C; Meyenhofer, MF; Shriver, K; Sharer, LR; Ou, CY; Rayfield, MA
Published Date
- November 1989
Published In
Volume / Issue
- 113 / 11
Start / End Page
- 1245 - 1249
PubMed ID
- 2684088
International Standard Serial Number (ISSN)
- 0003-9985
Language
- eng
Conference Location
- United States