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Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity.

Publication ,  Journal Article
Tei, L; Mazooz, G; Shellef, Y; Avni, R; Vandoorne, K; Barge, A; Kalchenko, V; Dewhirst, MW; Chaabane, L; Miragoli, L; Longo, D; Neeman, M; Aime, S
Published in: Contrast Media Mol Imaging
2010

Transglutaminases, including factor XIII and tissue transglutaminase, participate in multiple extracellular processes associated with remodeling of the extracellular matrix during wound repair, blood clotting, tumor progression and fibrosis of ischemic injuries. The aim of this work was to evaluate a novel substrate analog for transglutaminase optimized by molecular modeling calculations (DCCP16), which can serve for molecular imaging of transglutaminase activity by magnetic resonance imaging and by near-infrared imaging. Experimental data showed covalent binding of Gd-DCCP16 and DCCP16-IRIS Blue to human clots, to basement membrane components and to casein in purified systems as well as in three-dimensional multicellular spheroids. In vivo, DCCP16 showed enhancement with a prolonged retention in clots and tumors, demonstrating the ability to detect both factor XIII and tissue transglutaminase mediated covalent binding of the contrast material.

Duke Scholars

Published In

Contrast Media Mol Imaging

DOI

EISSN

1555-4317

Publication Date

2010

Volume

5

Issue

4

Start / End Page

213 / 222

Location

England

Related Subject Headings

  • Transglutaminases
  • Spheroids, Cellular
  • Peptides
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred Strains
  • Mice
  • Magnetic Resonance Imaging
  • Indoles
  • Humans
  • Fluorescent Dyes
 

Citation

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ICMJE
MLA
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Tei, L., Mazooz, G., Shellef, Y., Avni, R., Vandoorne, K., Barge, A., … Aime, S. (2010). Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity. Contrast Media Mol Imaging, 5(4), 213–222. https://doi.org/10.1002/cmmi.392
Tei, Lorenzo, Galit Mazooz, Yael Shellef, Reut Avni, Katrien Vandoorne, Alessandro Barge, Vyacheslav Kalchenko, et al. “Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity.Contrast Media Mol Imaging 5, no. 4 (2010): 213–22. https://doi.org/10.1002/cmmi.392.
Tei L, Mazooz G, Shellef Y, Avni R, Vandoorne K, Barge A, et al. Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity. Contrast Media Mol Imaging. 2010;5(4):213–22.
Tei, Lorenzo, et al. “Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity.Contrast Media Mol Imaging, vol. 5, no. 4, 2010, pp. 213–22. Pubmed, doi:10.1002/cmmi.392.
Tei L, Mazooz G, Shellef Y, Avni R, Vandoorne K, Barge A, Kalchenko V, Dewhirst MW, Chaabane L, Miragoli L, Longo D, Neeman M, Aime S. Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity. Contrast Media Mol Imaging. 2010;5(4):213–222.
Journal cover image

Published In

Contrast Media Mol Imaging

DOI

EISSN

1555-4317

Publication Date

2010

Volume

5

Issue

4

Start / End Page

213 / 222

Location

England

Related Subject Headings

  • Transglutaminases
  • Spheroids, Cellular
  • Peptides
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred Strains
  • Mice
  • Magnetic Resonance Imaging
  • Indoles
  • Humans
  • Fluorescent Dyes