Optical imaging of tumor hypoxia dynamics.

Published

Journal Article

The influence of the tumor microenvironment and hypoxia plays a significant role in determining cancer progression, treatment response, and treatment resistance. That the tumor microenvironment is highly heterogeneous with significant intratumor and intertumor variability presents a significant challenge in developing effective cancer therapies. Critical to understanding the role of the tumor microenvironment is the ability to dynamically quantify oxygen levels in the vasculature and tissue in order to elucidate the roles of oxygen supply and consumption, spatially and temporally. To this end, we describe the use of hyperspectral imaging to characterize hemoglobin absorption to quantify hemoglobin content and oxygen saturation, as well as dual emissive fluorescent∕phosphorescent boron nanoparticles, which serve as ratiometric indicators of tissue oxygen tension. Applying these techniques to a window-chamber tumor model illustrates the role of fluctuations in hemoglobin saturation in driving changes in tissue oxygenation, the two being significantly correlated (r = 0.77). Finally, a green-fluorescence-protein reporter for hypoxia inducible factor-1 (HIF-1) provides an endpoint for hypoxic stress in the tumor, which is used to demonstrate a significant association between tumor hypoxia dynamics and HIF-1 activity in an in vivo demonstration of the technique.

Full Text

Duke Authors

Cited Authors

  • Palmer, GM; Fontanella, AN; Zhang, G; Hanna, G; Fraser, CL; Dewhirst, MW

Published Date

  • November 2010

Published In

Volume / Issue

  • 15 / 6

Start / End Page

  • 066021 -

PubMed ID

  • 21198195

Pubmed Central ID

  • 21198195

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

Digital Object Identifier (DOI)

  • 10.1117/1.3523363

Language

  • eng

Conference Location

  • United States