Skip to main content

Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat.

Publication ,  Journal Article
Wetsel, WC; Culler, MD; Johnston, CA; Negro-Vilar, A
Published in: Mol Endocrinol
January 1988

The LHRH precursor is known to contain the decapeptide and a 56 amino acid peptide termed gonadotropin-releasing hormone-associated peptide (GAP). The purpose of our study was to characterize the proLHRH and its processed products from the cell body and fiber region and from the nerve terminal region of LHRH neurons. The median eminence (ME) and a tissue block containing the preoptic area and hypothalamus (POH) were dissected separately. Tissues were homogenized and peptides were separated according to mol wt. Three different LHRH antisera bound to one immunoreactive (IR) substance which eluted at approximately 1200 mol wt. Subsequently, this material coeluted with synthetic LHRH on a reversed-phase column as a single peak. There was approximately 1.6-fold more LHRH-like IR in the ME than in the POH. The four different GAP antisera recognized multiple mol wt forms of GAP-like IR at approximately 16,000 to 14,000, 8,200, 6,500, 3,500, and 2,800 mol wt. There were more of the high mol wt materials and less of the 6500 and lower mol wt materials in the POH than in the ME. The most abundant species in both regions was the 6500 mol wt form. This IR substance coeluted with synthetic rat GAP1-56 on a reversed-phase column as a single peak. These experiments demonstrate 1) that multiple IR forms of the LHRH prohormone exist in the POH of the rat and 2) that nerve terminals of the LHRH neurons contain LHRH, GAP1-56, and some lower mol wt GAP-like substances. These results provide the first information concerning the processing scheme for the LHRH prohormone in the rat brain.

Duke Scholars

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

January 1988

Volume

2

Issue

1

Start / End Page

22 / 31

Location

United States

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • Protein Processing, Post-Translational
  • Protein Precursors
  • Preoptic Area
  • Molecular Sequence Data
  • Median Eminence
  • Male
  • Hypothalamus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wetsel, W. C., Culler, M. D., Johnston, C. A., & Negro-Vilar, A. (1988). Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat. Mol Endocrinol, 2(1), 22–31. https://doi.org/10.1210/mend-2-1-22
Wetsel, W. C., M. D. Culler, C. A. Johnston, and A. Negro-Vilar. “Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat.Mol Endocrinol 2, no. 1 (January 1988): 22–31. https://doi.org/10.1210/mend-2-1-22.
Wetsel WC, Culler MD, Johnston CA, Negro-Vilar A. Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat. Mol Endocrinol. 1988 Jan;2(1):22–31.
Wetsel, W. C., et al. “Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat.Mol Endocrinol, vol. 2, no. 1, Jan. 1988, pp. 22–31. Pubmed, doi:10.1210/mend-2-1-22.
Wetsel WC, Culler MD, Johnston CA, Negro-Vilar A. Processing of the luteinizing hormone-releasing hormone precursor in the preoptic area and hypothalamus of the rat. Mol Endocrinol. 1988 Jan;2(1):22–31.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

January 1988

Volume

2

Issue

1

Start / End Page

22 / 31

Location

United States

Related Subject Headings

  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • Protein Processing, Post-Translational
  • Protein Precursors
  • Preoptic Area
  • Molecular Sequence Data
  • Median Eminence
  • Male
  • Hypothalamus