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Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation.

Publication ,  Journal Article
Battaini, F; Pascale, A; Lucchi, L; Racchi, M; Bergamaschi, S; Parenti, M; Wetsel, WC; Govoni, S; Trabucchi, M
Published in: Biochem Biophys Res Commun
September 30, 1994

Protein, mRNA and activity levels of the calcium-independent protein kinase C (nPKC) isoenzymes were examined in NG108-15 cells. Western blot analyses reveal that proliferating NG 108-15 cells express the delta, epsilon, and eta, but not the theta species. The atypical species PKC zeta was also detected. Differentiation of these cells with dibutyryl cAMP was associated with increase in the levels of PKC epsilon, with no significant changes in its steady-state mRNA levels. The levels of the other isoforms were not altered by the differentiated state. Similarly, no changes in nPKC activity were discerned in either the soluble or particulate fractions when histone or other proteins were used as substrates. These data suggest that the PKC epsilon isoform may be important for the production and maintenance of the differentiated state in NG 108-15 cells.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 30, 1994

Volume

203

Issue

3

Start / End Page

1423 / 1431

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Rats
  • RNA, Messenger
  • Protein Kinase C
  • Neuroblastoma
  • Mice
  • Kinetics
  • Isoenzymes
  • Hybrid Cells
  • Glioma
 

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Battaini, F., Pascale, A., Lucchi, L., Racchi, M., Bergamaschi, S., Parenti, M., … Trabucchi, M. (1994). Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation. Biochem Biophys Res Commun, 203(3), 1423–1431. https://doi.org/10.1006/bbrc.1994.2344
Battaini, F., A. Pascale, L. Lucchi, M. Racchi, S. Bergamaschi, M. Parenti, W. C. Wetsel, S. Govoni, and M. Trabucchi. “Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation.Biochem Biophys Res Commun 203, no. 3 (September 30, 1994): 1423–31. https://doi.org/10.1006/bbrc.1994.2344.
Battaini F, Pascale A, Lucchi L, Racchi M, Bergamaschi S, Parenti M, et al. Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation. Biochem Biophys Res Commun. 1994 Sep 30;203(3):1423–31.
Battaini, F., et al. “Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation.Biochem Biophys Res Commun, vol. 203, no. 3, Sept. 1994, pp. 1423–31. Pubmed, doi:10.1006/bbrc.1994.2344.
Battaini F, Pascale A, Lucchi L, Racchi M, Bergamaschi S, Parenti M, Wetsel WC, Govoni S, Trabucchi M. Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation. Biochem Biophys Res Commun. 1994 Sep 30;203(3):1423–1431.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 30, 1994

Volume

203

Issue

3

Start / End Page

1423 / 1431

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Rats
  • RNA, Messenger
  • Protein Kinase C
  • Neuroblastoma
  • Mice
  • Kinetics
  • Isoenzymes
  • Hybrid Cells
  • Glioma