Mice deficient for the vesicular acetylcholine transporter are myasthenic and have deficits in object and social recognition.

Journal Article

An important step for cholinergic transmission involves the vesicular storage of acetylcholine (ACh), a process mediated by the vesicular acetylcholine transporter (VAChT). In order to understand the physiological roles of the VAChT, we developed a genetically altered strain of mice with reduced expression of this transporter. Heterozygous and homozygous VAChT knockdown mice have a 45% and 65% decrease in VAChT protein expression, respectively. VAChT deficiency alters synaptic vesicle filling and affects ACh release. Whereas VAChT homozygous mutant mice demonstrate major neuromuscular deficits, VAChT heterozygous mice appear normal in that respect and could be used for analysis of central cholinergic function. Behavioral analyses revealed that aversive learning and memory are not altered in mutant mice; however, performance in cognitive tasks involving object and social recognition is severely impaired. These observations suggest a critical role of VAChT in the regulation of ACh release and physiological functions in the peripheral and central nervous system.

Full Text

Duke Authors

Cited Authors

  • Prado, VF; Martins-Silva, C; de Castro, BM; Lima, RF; Barros, DM; Amaral, E; Ramsey, AJ; Sotnikova, TD; Ramirez, MR; Kim, H-G; Rossato, JI; Koenen, J; Quan, H; Cota, VR; Moraes, MFD; Gomez, MV; Guatimosim, C; Wetsel, WC; Kushmerick, C; Pereira, GS; Gainetdinov, RR; Izquierdo, I; Caron, MG; Prado, MAM

Published Date

  • September 7, 2006

Published In

Volume / Issue

  • 51 / 5

Start / End Page

  • 601 - 612

PubMed ID

  • 16950158

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2006.08.005

Language

  • eng

Conference Location

  • United States