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Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization.

Publication ,  Journal Article
Chen, Q; Xiong, X; Lee, TH; Liu, Y; Wetsel, WC; Zhang, X
Published in: J Neurochem
November 2008

Behavioral sensitization of psychostimulants was accompanied by alterations in a variety of biochemical molecules in different brain regions. However, which change is actually related to drug-produced sensitization lacks of accurate clarification. In this study, we investigated the role of integrin-linked kinase (ILK) in both the induction and expression of cocaine sensitization. Conditional inhibition of ILK expression was established in the nucleus accumbens (NAc) core by microinjecting recombinant adeno-associated virus-carrying, tetracycline-on-regulated small interfering RNA which reversed the chronic cocaine-induced psychomotor sensitization, as well as the changes in protein kinase B Ser473 phosphorylation, dendritic density, and dendritic spine numbers locally. Importantly, the reversed psychomotor sensitization did not recover after cessation of the silencing for 8 days. We also demonstrated that inhibition of ILK expression pre- and during-chronic cocaine treatments blocked the induction of cocaine psychomotor sensitization and abolished the stimulant effect of cocaine on ILK expression. In contrast, inhibition of ILK expression in the NAc core has no significant effect on cocaine-induced stereotypical behaviors. This concludes that ILK is involved in cocaine sensitization with the earlier induction and later expression functioning as a kinase to regulate protein kinase B Ser473 phosphorylation and a scaffolding protein to regulate the reorganization of the NAc spine morphology.

Duke Scholars

Published In

J Neurochem

DOI

EISSN

1471-4159

Publication Date

November 2008

Volume

107

Issue

3

Start / End Page

679 / 689

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Small Interfering
  • Proto-Oncogene Proteins c-akt
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Male
  • Fluorescent Antibody Technique
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, Q., Xiong, X., Lee, T. H., Liu, Y., Wetsel, W. C., & Zhang, X. (2008). Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization. J Neurochem, 107(3), 679–689. https://doi.org/10.1111/j.1471-4159.2008.05619.x
Chen, Qiang, Xueying Xiong, Tong H. Lee, Yingmiao Liu, William C. Wetsel, and Xiuwu Zhang. “Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization.J Neurochem 107, no. 3 (November 2008): 679–89. https://doi.org/10.1111/j.1471-4159.2008.05619.x.
Chen Q, Xiong X, Lee TH, Liu Y, Wetsel WC, Zhang X. Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization. J Neurochem. 2008 Nov;107(3):679–89.
Chen, Qiang, et al. “Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization.J Neurochem, vol. 107, no. 3, Nov. 2008, pp. 679–89. Pubmed, doi:10.1111/j.1471-4159.2008.05619.x.
Chen Q, Xiong X, Lee TH, Liu Y, Wetsel WC, Zhang X. Neural plasticity and addiction: integrin-linked kinase and cocaine behavioral sensitization. J Neurochem. 2008 Nov;107(3):679–689.
Journal cover image

Published In

J Neurochem

DOI

EISSN

1471-4159

Publication Date

November 2008

Volume

107

Issue

3

Start / End Page

679 / 689

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Small Interfering
  • Proto-Oncogene Proteins c-akt
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Male
  • Fluorescent Antibody Technique