UV absorption complicates PCR decontamination.
Journal Article (Journal Article)
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
Full Text
Duke Authors
Cited Authors
- Frothingham, R; Blitchington, RB; Lee, DH; Greene, RC; Wilson, KH
Published Date
- August 1992
Published In
Volume / Issue
- 13 / 2
Start / End Page
- 208 - 210
PubMed ID
- 1389153
International Standard Serial Number (ISSN)
- 0736-6205
Language
- eng
Conference Location
- England