UV absorption complicates PCR decontamination.

Journal Article (Journal Article)

UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.

Full Text

Duke Authors

Cited Authors

  • Frothingham, R; Blitchington, RB; Lee, DH; Greene, RC; Wilson, KH

Published Date

  • August 1992

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 208 - 210

PubMed ID

  • 1389153

International Standard Serial Number (ISSN)

  • 0736-6205


  • eng

Conference Location

  • England