UV absorption complicates PCR decontamination.
Publication
, Journal Article
Frothingham, R; Blitchington, RB; Lee, DH; Greene, RC; Wilson, KH
Published in: Biotechniques
August 1992
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
Duke Scholars
Published In
Biotechniques
ISSN
0736-6205
Publication Date
August 1992
Volume
13
Issue
2
Start / End Page
208 / 210
Location
England
Related Subject Headings
- Ultraviolet Rays
- Polymerase Chain Reaction
- Molecular Sequence Data
- DNA
- Bioinformatics
- Base Sequence
- Artifacts
- 31 Biological sciences
- 10 Technology
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Frothingham, R., Blitchington, R. B., Lee, D. H., Greene, R. C., & Wilson, K. H. (1992). UV absorption complicates PCR decontamination. Biotechniques, 13(2), 208–210.
Frothingham, R., R. B. Blitchington, D. H. Lee, R. C. Greene, and K. H. Wilson. “UV absorption complicates PCR decontamination.” Biotechniques 13, no. 2 (August 1992): 208–10.
Frothingham R, Blitchington RB, Lee DH, Greene RC, Wilson KH. UV absorption complicates PCR decontamination. Biotechniques. 1992 Aug;13(2):208–10.
Frothingham, R., et al. “UV absorption complicates PCR decontamination.” Biotechniques, vol. 13, no. 2, Aug. 1992, pp. 208–10.
Frothingham R, Blitchington RB, Lee DH, Greene RC, Wilson KH. UV absorption complicates PCR decontamination. Biotechniques. 1992 Aug;13(2):208–210.
Published In
Biotechniques
ISSN
0736-6205
Publication Date
August 1992
Volume
13
Issue
2
Start / End Page
208 / 210
Location
England
Related Subject Headings
- Ultraviolet Rays
- Polymerase Chain Reaction
- Molecular Sequence Data
- DNA
- Bioinformatics
- Base Sequence
- Artifacts
- 31 Biological sciences
- 10 Technology
- 06 Biological Sciences