Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification.

Journal Article (Journal Article)

Ribosomal RNA sequences are useful for establishing phylogenetic relationships, for oligonucleotide probes and for characterization of uncultured organisms. We describe rapid ribosomal DNA sequencing using PCR with transcript sequencing. Nucleic acid specificity at three steps (amplification, transcription and sequencing) eliminated the need for nucleic acid extraction or purification. Sequence was obtained from a crude lysate from a single colony of bacteria. The basic sequencing method should be adaptable to provide rapid sequence information in a wide variety of applications.

Full Text

Duke Authors

Cited Authors

  • Frothingham, R; Allen, RL; Wilson, KH

Published Date

  • July 1991

Published In

Volume / Issue

  • 11 / 1

Start / End Page

  • 40 - 44

PubMed ID

  • 1720002

International Standard Serial Number (ISSN)

  • 0736-6205


  • eng

Conference Location

  • England