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Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis.

Publication ,  Journal Article
Duncan, AJ; Carman, RJ; Olsen, GJ; Wilson, KH
Published in: Int J Syst Bacteriol
April 1993

The small-subunit rRNA (16S rRNA) sequence of Tyzzer's bacillus (also known as "Bacillus piliformis") was elucidated by using the polymerase chain reaction followed by reverse transcriptase sequencing. By using maximum-likelihood analysis, a phylogenetic tree was constructed from this and other 16S rRNA sequences available from the first release of the Ribosomal Database Project (G. J. Olsen, R. Overbeek, N. Larsen, T. L. Marsh, M. J. McCaughey, M. A. Maciukenas, W.-M. Kuan, T. J. Macke, Y. Xing, and C. R. Woese, Nucleic Acids Res. 20:2199-2200, 1992). Tyzzer's bacillus grouped with a specific set of anaerobic bacteria, most of which are Clostridium spp. The closest identified relatives are Clostridium coccoides, Clostridium oroticum, Clostridium clostridiiforme, Clostridium symbiosum, and Streptococcus hansenii. Clostridium amino-valericum and "Acetitomaculum ruminis" are also solidly allied with this ensemble. We propose that Tyzzer's bacillus be reclassified as Clostridium piliforme on the basis of its 16S rRNA sequence.

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Published In

Int J Syst Bacteriol

DOI

ISSN

0020-7713

Publication Date

April 1993

Volume

43

Issue

2

Start / End Page

314 / 318

Location

England

Related Subject Headings

  • Rodent Diseases
  • RNA, Ribosomal, 16S
  • RNA, Bacterial
  • Phylogeny
  • Molecular Sequence Data
  • Microbiology
  • Mice
  • Clostridium
  • Biological Evolution
  • Base Sequence
 

Citation

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Duncan, A. J., Carman, R. J., Olsen, G. J., & Wilson, K. H. (1993). Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis. Int J Syst Bacteriol, 43(2), 314–318. https://doi.org/10.1099/00207713-43-2-314
Duncan, A. J., R. J. Carman, G. J. Olsen, and K. H. Wilson. “Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis.Int J Syst Bacteriol 43, no. 2 (April 1993): 314–18. https://doi.org/10.1099/00207713-43-2-314.
Duncan AJ, Carman RJ, Olsen GJ, Wilson KH. Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis. Int J Syst Bacteriol. 1993 Apr;43(2):314–8.
Duncan, A. J., et al. “Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis.Int J Syst Bacteriol, vol. 43, no. 2, Apr. 1993, pp. 314–18. Pubmed, doi:10.1099/00207713-43-2-314.
Duncan AJ, Carman RJ, Olsen GJ, Wilson KH. Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis. Int J Syst Bacteriol. 1993 Apr;43(2):314–318.

Published In

Int J Syst Bacteriol

DOI

ISSN

0020-7713

Publication Date

April 1993

Volume

43

Issue

2

Start / End Page

314 / 318

Location

England

Related Subject Headings

  • Rodent Diseases
  • RNA, Ribosomal, 16S
  • RNA, Bacterial
  • Phylogeny
  • Molecular Sequence Data
  • Microbiology
  • Mice
  • Clostridium
  • Biological Evolution
  • Base Sequence