High-resolution in vivo imaging of fluorescent proteins using window chamber models.

Journal Article (Journal Article)

Fluorescent proteins enable in vivo characterization of a wide and growing array of morphological and functional biomarkers. To fully capitalize on the spatial and temporal information afforded by these reporter proteins, a method for imaging these proteins at high resolution longitudinally is required. This chapter describes the use of window chamber models as a means of imaging fluorescent proteins and other optical parameters. Such models essentially involve surgically implanting a window through which tumor or normal tissue can be imaged using existing microscopy techniques. This enables acquisition of high-quality images down to the cellular or subcellular scale, exploiting the diverse array of optical contrast mechanisms, while also maintaining the native microenvironment of the tissue of interest. This makes these techniques applicable to a wide array of problems in the biomedical sciences.

Full Text

Duke Authors

Cited Authors

  • Palmer, GM; Fontanella, AN; Shan, S; Dewhirst, MW

Published Date

  • 2012

Published In

Volume / Issue

  • 872 /

Start / End Page

  • 31 - 50

PubMed ID

  • 22700402

Pubmed Central ID

  • PMC3736593

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-61779-797-2_3


  • eng

Conference Location

  • United States