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High-resolution in vivo imaging of fluorescent proteins using window chamber models.

Publication ,  Journal Article
Palmer, GM; Fontanella, AN; Shan, S; Dewhirst, MW
Published in: Methods Mol Biol
2012

Fluorescent proteins enable in vivo characterization of a wide and growing array of morphological and functional biomarkers. To fully capitalize on the spatial and temporal information afforded by these reporter proteins, a method for imaging these proteins at high resolution longitudinally is required. This chapter describes the use of window chamber models as a means of imaging fluorescent proteins and other optical parameters. Such models essentially involve surgically implanting a window through which tumor or normal tissue can be imaged using existing microscopy techniques. This enables acquisition of high-quality images down to the cellular or subcellular scale, exploiting the diverse array of optical contrast mechanisms, while also maintaining the native microenvironment of the tissue of interest. This makes these techniques applicable to a wide array of problems in the biomedical sciences.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2012

Volume

872

Start / End Page

31 / 50

Location

United States

Related Subject Headings

  • Neoplasms
  • Green Fluorescent Proteins
  • Fluorescence
  • Diagnostic Imaging
  • Developmental Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

APA
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ICMJE
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Palmer, G. M., Fontanella, A. N., Shan, S., & Dewhirst, M. W. (2012). High-resolution in vivo imaging of fluorescent proteins using window chamber models. Methods Mol Biol, 872, 31–50. https://doi.org/10.1007/978-1-61779-797-2_3
Palmer, Gregory M., Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst. “High-resolution in vivo imaging of fluorescent proteins using window chamber models.Methods Mol Biol 872 (2012): 31–50. https://doi.org/10.1007/978-1-61779-797-2_3.
Palmer GM, Fontanella AN, Shan S, Dewhirst MW. High-resolution in vivo imaging of fluorescent proteins using window chamber models. Methods Mol Biol. 2012;872:31–50.
Palmer, Gregory M., et al. “High-resolution in vivo imaging of fluorescent proteins using window chamber models.Methods Mol Biol, vol. 872, 2012, pp. 31–50. Pubmed, doi:10.1007/978-1-61779-797-2_3.
Palmer GM, Fontanella AN, Shan S, Dewhirst MW. High-resolution in vivo imaging of fluorescent proteins using window chamber models. Methods Mol Biol. 2012;872:31–50.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2012

Volume

872

Start / End Page

31 / 50

Location

United States

Related Subject Headings

  • Neoplasms
  • Green Fluorescent Proteins
  • Fluorescence
  • Diagnostic Imaging
  • Developmental Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences