A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors.

Published

Journal Article

This study describes a quantitative method to measure the magnitude and distribution of gene expression following local delivery of an adenoviral vector containing the human sodium iodide symporter (hNIS) reporter gene into the canine prostate. Following systemic administration of Na(99m)TcO(4), autoradiographs of prostate sections depicting hNIS-dependent (99m)TcO(4)(-) uptake were digitized and stacked to produce a three-dimensional reconstruction of gene expression. Frequency histograms reflecting hNIS gene expression magnitude and volume were used to quantify hNIS function. The method demonstrated submillimeter resolution allowing for precise measurements of gene expression magnitude and volume in vivo. The method developed here could be applied to other reporter gene systems in which the readout can be digitized from thin tissue sections.

Full Text

Duke Authors

Cited Authors

  • Barton, KN; Xia, X; Yan, H; Stricker, H; Heisey, G; Yin, F-F; Nagaraja, TN; Zhu, G; Kolozsvary, A; Fenstermacher, JD; Lu, M; Kim, JH; Freytag, SO; Brown, SL

Published Date

  • April 2004

Published In

Volume / Issue

  • 9 / 4

Start / End Page

  • 625 - 631

PubMed ID

  • 15093193

Pubmed Central ID

  • 15093193

Electronic International Standard Serial Number (EISSN)

  • 1525-0024

International Standard Serial Number (ISSN)

  • 1525-0016

Digital Object Identifier (DOI)

  • 10.1016/j.ymthe.2004.01.011

Language

  • eng