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IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses.

Publication ,  Journal Article
Moreno, AA; Mukhtar, MS; Blanco, F; Boatwright, JL; Moreno, I; Jordan, MR; Chen, Y; Brandizzi, F; Dong, X; Orellana, A; Pajerowska Mukhtar, KM
Published in: PloS one
February 2012

Endoplasmic reticulum (ER)-mediated protein secretion and quality control have been shown to play an important role in immune responses in both animals and plants. In mammals, the ER membrane-located IRE1 kinase/endoribonuclease, a key regulator of unfolded protein response (UPR), is required for plasma cell development to accommodate massive secretion of immunoglobulins. Plant cells can secrete the so-called pathogenesis-related (PR) proteins with antimicrobial activities upon pathogen challenge. However, whether IRE1 plays any role in plant immunity is not known. Arabidopsis thaliana has two copies of IRE1, IRE1a and IRE1b. Here, we show that both IRE1a and IRE1b are transcriptionally induced during chemically-induced ER stress, bacterial pathogen infection and treatment with the immune signal salicylic acid (SA). However, we found that IRE1a plays a predominant role in the secretion of PR proteins upon SA treatment. Consequently, the ire1a mutant plants show enhanced susceptibility to a bacterial pathogen and are deficient in establishing systemic acquired resistance (SAR), whereas ire1b is unaffected in these responses. We further demonstrate that the immune deficiency in ire1a is due to a defect in SA- and pathogen-triggered, IRE1-mediated cytoplasmic splicing of the bZIP60 mRNA, which encodes a transcription factor involved in the expression of UPR-responsive genes. Consistently, IRE1a is preferentially required for bZIP60 splicing upon pathogen infection, while IRE1b plays a major role in bZIP60 processing upon Tunicamycin (Tm)-induced stress. We also show that SA-dependent induction of UPR-responsive genes is altered in the bzip60 mutant resulting in a moderate susceptibility to a bacterial pathogen. These results indicate that the IRE1/bZIP60 branch of UPR is a part of the plant response to pathogens for which the two Arabidopsis IRE1 isoforms play only partially overlapping roles and that IRE1 has both bZIP60-dependent and bZIP60-independent functions in plant immunity.

Duke Scholars

Published In

PloS one

DOI

ISSN

1932-6203

Publication Date

February 2012

Volume

7

Issue

2

Start / End Page

e31944

Related Subject Headings

  • Unfolded Protein Response
  • Transcriptional Activation
  • Transcription Factors
  • Protein Kinases
  • Protein Isoforms
  • Plant Immunity
  • General Science & Technology
  • Endoribonucleases
  • Basic-Leucine Zipper Transcription Factors
  • Arabidopsis Proteins
 

Citation

APA
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Moreno, A. A., Mukhtar, M. S., Blanco, F., Boatwright, J. L., Moreno, I., Jordan, M. R., … Pajerowska Mukhtar, K. M. (2012). IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses. PloS One, 7(2), e31944. https://doi.org/10.1371/journal.pone.0031944
Moreno, A. A., M. S. Mukhtar, F. Blanco, J. L. Boatwright, I. Moreno, M. R. Jordan, Y. Chen, et al. “IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses.PloS One 7, no. 2 (February 2012): e31944. https://doi.org/10.1371/journal.pone.0031944.
Moreno AA, Mukhtar MS, Blanco F, Boatwright JL, Moreno I, Jordan MR, et al. IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses. PloS one. 2012 Feb;7(2):e31944.
Moreno, A. A., et al. “IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses.PloS One, vol. 7, no. 2, Feb. 2012, p. e31944. Manual, doi:10.1371/journal.pone.0031944.
Moreno AA, Mukhtar MS, Blanco F, Boatwright JL, Moreno I, Jordan MR, Chen Y, Brandizzi F, Dong X, Orellana A, Pajerowska Mukhtar KM. IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses. PloS one. 2012 Feb;7(2):e31944.

Published In

PloS one

DOI

ISSN

1932-6203

Publication Date

February 2012

Volume

7

Issue

2

Start / End Page

e31944

Related Subject Headings

  • Unfolded Protein Response
  • Transcriptional Activation
  • Transcription Factors
  • Protein Kinases
  • Protein Isoforms
  • Plant Immunity
  • General Science & Technology
  • Endoribonucleases
  • Basic-Leucine Zipper Transcription Factors
  • Arabidopsis Proteins