Molecular identification, cloning and characterization of transmitted/founder HIV-1 subtype A, D and A/D infectious molecular clones.
We report the molecular identification, cloning and initial biological characterization of 12 full-length HIV-1 subtype A, D and A/D recombinant transmitted/founder (T/F) genomes. T/F genomes contained intact canonical open reading frames and all T/F viruses were replication competent in primary human T-cells, although subtype D virus replication was more efficient (p<0.05). All 12 viruses utilized CCR5 but not CXCR4 as a co-receptor for entry and exhibited a neutralization profile typical of tier 2 primary virus strains, with significant differences observed between subtype A and D viruses with respect to sensitivity to monoclonal antibodies VRC01, PG9 and PG16 and polyclonal subtype C anti-HIV IgG (p<0.05 for each). The present report doubles the number of T/F HIV-1 clones available for pathogenesis and vaccine research and extends their representation to include subtypes A, B, C and D.
Baalwa, J; Wang, S; Parrish, NF; Decker, JM; Keele, BF; Learn, GH; Yue, L; Ruzagira, E; Ssemwanga, D; Kamali, A; Amornkul, PN; Price, MA; Kappes, JC; Karita, E; Kaleebu, P; Sanders, E; Gilmour, J; Allen, S; Hunter, E; Montefiori, DC; Haynes, BF; Cormier, E; Hahn, BH; Shaw, GM
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