Cowpox virus induces interleukin-10 both in vitro and in vivo.

Published

Journal Article

Cowpox virus infection induces interleukin-10 (IL-10) production from mouse bone marrow-derived dendritic cells (BMDCs) or cells of the mouse macrophage line (RAW264.7) at about 1800 pg/ml, whereas infections with vaccinia virus (strains WR or MVA) induced much less IL-10. Similarly, in vivo, IL-10 levels in bronchoalveolar lavage fluids of mice infected with cowpox virus were significantly higher than those after vaccinia virus infection. However, after intranasal cowpox virus infection, although dendritic and T-cell accumulations in the lungs of IL-10 deficient mice were greater than those in wild-type mice, weight-loss and viral burdens were not significantly different. IL-10 deficient mice were more susceptible than wild-type mice to re-infection with cowpox virus even though titers of neutralizing antibodies and virus-specific CD8 T cells were similar between IL-10 deficient and wild-type mice. Greater bronchopneumonia in IL-10 deficient mice than wild-type mice suggests that IL-10 contributes to the suppression of immunopathology in the lungs.

Full Text

Duke Authors

Cited Authors

  • Spesock, AH; Barefoot, BE; Ray, CA; Kenan, DJ; Gunn, MD; Ramsburg, EA; Pickup, DJ

Published Date

  • August 2011

Published In

Volume / Issue

  • 417 / 1

Start / End Page

  • 87 - 97

PubMed ID

  • 21658738

Pubmed Central ID

  • 21658738

Electronic International Standard Serial Number (EISSN)

  • 2514-4138

International Standard Serial Number (ISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2011.05.010

Language

  • eng