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Cowpox virus induces interleukin-10 both in vitro and in vivo.

Publication ,  Journal Article
Spesock, AH; Barefoot, BE; Ray, CA; Kenan, DJ; Gunn, MD; Ramsburg, EA; Pickup, DJ
Published in: Virology
August 15, 2011

Cowpox virus infection induces interleukin-10 (IL-10) production from mouse bone marrow-derived dendritic cells (BMDCs) or cells of the mouse macrophage line (RAW264.7) at about 1800 pg/ml, whereas infections with vaccinia virus (strains WR or MVA) induced much less IL-10. Similarly, in vivo, IL-10 levels in bronchoalveolar lavage fluids of mice infected with cowpox virus were significantly higher than those after vaccinia virus infection. However, after intranasal cowpox virus infection, although dendritic and T-cell accumulations in the lungs of IL-10 deficient mice were greater than those in wild-type mice, weight-loss and viral burdens were not significantly different. IL-10 deficient mice were more susceptible than wild-type mice to re-infection with cowpox virus even though titers of neutralizing antibodies and virus-specific CD8 T cells were similar between IL-10 deficient and wild-type mice. Greater bronchopneumonia in IL-10 deficient mice than wild-type mice suggests that IL-10 contributes to the suppression of immunopathology in the lungs.

Duke Scholars

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

August 15, 2011

Volume

417

Issue

1

Start / End Page

87 / 97

Location

United States

Related Subject Headings

  • Weight Loss
  • Virology
  • T-Lymphocytes
  • Mutation
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Lung
  • Interleukin-10
  • Gene Expression Regulation
 

Citation

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MLA
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Spesock, A. H., Barefoot, B. E., Ray, C. A., Kenan, D. J., Gunn, M. D., Ramsburg, E. A., & Pickup, D. J. (2011). Cowpox virus induces interleukin-10 both in vitro and in vivo. Virology, 417(1), 87–97. https://doi.org/10.1016/j.virol.2011.05.010
Spesock, April H., Brice E. Barefoot, Caroline A. Ray, Daniel J. Kenan, Michael D. Gunn, Elizabeth A. Ramsburg, and David J. Pickup. “Cowpox virus induces interleukin-10 both in vitro and in vivo.Virology 417, no. 1 (August 15, 2011): 87–97. https://doi.org/10.1016/j.virol.2011.05.010.
Spesock AH, Barefoot BE, Ray CA, Kenan DJ, Gunn MD, Ramsburg EA, et al. Cowpox virus induces interleukin-10 both in vitro and in vivo. Virology. 2011 Aug 15;417(1):87–97.
Spesock, April H., et al. “Cowpox virus induces interleukin-10 both in vitro and in vivo.Virology, vol. 417, no. 1, Aug. 2011, pp. 87–97. Pubmed, doi:10.1016/j.virol.2011.05.010.
Spesock AH, Barefoot BE, Ray CA, Kenan DJ, Gunn MD, Ramsburg EA, Pickup DJ. Cowpox virus induces interleukin-10 both in vitro and in vivo. Virology. 2011 Aug 15;417(1):87–97.
Journal cover image

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

August 15, 2011

Volume

417

Issue

1

Start / End Page

87 / 97

Location

United States

Related Subject Headings

  • Weight Loss
  • Virology
  • T-Lymphocytes
  • Mutation
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Lung
  • Interleukin-10
  • Gene Expression Regulation