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PF4/heparin complexes are T cell-dependent antigens.

Publication ,  Journal Article
Suvarna, S; Rauova, L; McCracken, EKE; Goss, CM; Sachais, BS; McKenzie, SE; Reilly, MP; Gunn, MD; Cines, DB; Poncz, M; Arepally, G
Published in: Blood
August 1, 2005

Heparin-induced thrombocytopenia (HIT) is a life-threatening, thrombotic disorder associated with development of anti-platelet factor 4 (anti-PF4)/heparin autoantibodies. Little is known about the antigenic and cellular requirements that initiate the immune response to these complexes. To begin to delineate mechanisms of autoantibody formation in HIT, we studied the immunizing effects of murine PF4 (mPF4)/heparin in mice with and without thymic function. Euthymic mice were injected with mPF4/heparin complexes, mPF4, heparin, or buffer. Mice injected with mPF4/heparin, but not mPF4 or heparin alone, developed heparin-dependent autoantibodies that shared serologic and functional characteristics of human HIT antibodies, including preferential binding to mPF4/heparin complexes and causing heparin- and FcRgammaIIA-dependent platelet activation. In contrast, athymic mice did not develop HIT-like antibodies. Taken together, these studies establish that PF4/heparin complexes are highly immunogenic and elicit self-reacting anti-PF4/heparin antibodies in a T cell-dependent manner.

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Published In

Blood

DOI

ISSN

0006-4971

Publication Date

August 1, 2005

Volume

106

Issue

3

Start / End Page

929 / 931

Location

United States

Related Subject Headings

  • Thymus Gland
  • Thrombocytopenia
  • T-Lymphocytes
  • Platelet Factor 4
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunization
  • Heparin
  • Autoantibodies
 

Citation

APA
Chicago
ICMJE
MLA
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Suvarna, S., Rauova, L., McCracken, E. K. E., Goss, C. M., Sachais, B. S., McKenzie, S. E., … Arepally, G. (2005). PF4/heparin complexes are T cell-dependent antigens. Blood, 106(3), 929–931. https://doi.org/10.1182/blood-2004-12-4955
Suvarna, Shayela, Lubica Rauova, Emily K. E. McCracken, Christina M. Goss, Bruce S. Sachais, Steven E. McKenzie, Michael P. Reilly, et al. “PF4/heparin complexes are T cell-dependent antigens.Blood 106, no. 3 (August 1, 2005): 929–31. https://doi.org/10.1182/blood-2004-12-4955.
Suvarna S, Rauova L, McCracken EKE, Goss CM, Sachais BS, McKenzie SE, et al. PF4/heparin complexes are T cell-dependent antigens. Blood. 2005 Aug 1;106(3):929–31.
Suvarna, Shayela, et al. “PF4/heparin complexes are T cell-dependent antigens.Blood, vol. 106, no. 3, Aug. 2005, pp. 929–31. Pubmed, doi:10.1182/blood-2004-12-4955.
Suvarna S, Rauova L, McCracken EKE, Goss CM, Sachais BS, McKenzie SE, Reilly MP, Gunn MD, Cines DB, Poncz M, Arepally G. PF4/heparin complexes are T cell-dependent antigens. Blood. 2005 Aug 1;106(3):929–931.

Published In

Blood

DOI

ISSN

0006-4971

Publication Date

August 1, 2005

Volume

106

Issue

3

Start / End Page

929 / 931

Location

United States

Related Subject Headings

  • Thymus Gland
  • Thrombocytopenia
  • T-Lymphocytes
  • Platelet Factor 4
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunization
  • Heparin
  • Autoantibodies