CD4 T follicular helper cell dynamics during SIV infection.

Published

Journal Article

CD4 T follicular helper (TFH) cells interact with and stimulate the generation of antigen-specific B cells. TFH cell interaction with B cells correlates with production of SIV-specific immunoglobulins. However, the fate of TFH cells and their participation in SIV-induced antibody production is not well understood. We investigated the phenotype, function, location, and molecular signature of TFH cells in rhesus macaques. Similar to their human counterparts, TFH cells in rhesus macaques represented a heterogeneous population with respect to cytokine function. In a highly differentiated subpopulation of TFH cells, characterized by CD150lo expression, production of Th1 cytokines was compromised while IL-4 production was augmented, and cells exhibited decreased survival, cycling, and trafficking capacity. TFH cells exhibited a distinct gene profile that was markedly altered by SIV infection. TFH cells were infected by SIV; yet, in some animals, these cells actually accumulated during chronic SIV infection. Generalized immune activation and increased IL-6 production helped drive TFH differentiation during SIV infection. Accumulation of TFH cells was associated with increased frequency of activated germinal center B cells and SIV-specific antibodies. Therefore, chronic SIV does not disturb the ability of TFH cells to help B cell maturation and production of SIV-specific immunoglobulins.

Full Text

Duke Authors

Cited Authors

  • Petrovas, C; Yamamoto, T; Gerner, MY; Boswell, KL; Wloka, K; Smith, EC; Ambrozak, DR; Sandler, NG; Timmer, KJ; Sun, X; Pan, L; Poholek, A; Rao, SS; Brenchley, JM; Alam, SM; Tomaras, GD; Roederer, M; Douek, DC; Seder, RA; Germain, RN; Haddad, EK; Koup, RA

Published Date

  • September 2012

Published In

Volume / Issue

  • 122 / 9

Start / End Page

  • 3281 - 3294

PubMed ID

  • 22922258

Pubmed Central ID

  • 22922258

Electronic International Standard Serial Number (EISSN)

  • 1558-8238

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/JCI63039

Language

  • eng