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Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary.

Publication ,  Journal Article
Meyer, JN; Volz, DC; Freedman, JH; Di Giulio, RT
Published in: Aquatic toxicology (Amsterdam, Netherlands)
July 2005

Fundulus heteroclitus (Atlantic killifish, mummichog) from a highly contaminated site on the Elizabeth River (VA, USA) are resistant to the toxicity of sediment from the site. However, the mechanistic changes that confer resistance to the toxicity are not yet well understood. We utilized differential display to identify mRNAs that are differentially expressed in hepatic tissue of male and female killifish from the Elizabeth River environment, compared to killifish from a non-contaminated reference site, King's Creek (VA, USA). Seventy-four differentially expressed mRNAs were initially identified (including sex and population-specific differences), and 65 of these were isolated and sequenced. A reverse northern blot array constructed of these cDNAs (plus an additional 15 previously sequenced mRNAs of interest) was used to confirm and quantify expression differences. High interindividual variability was observed in mRNA expression, but multiple differentially expressed mRNAs were identified, including 11 population-specific differences occurring in both sexes, 24 population-specific differences occurring in only one sex, and 22 sex-specific differences. Many of these differentially expressed mRNAs were novel, or not previously hypothesized to play a role in response to contaminant exposure. In addition, the results indicate that the effect of contaminated sediment exposure on the expression of a large proportion of the differentially expressed mRNAs was dependent on the sex of the fish.

Duke Scholars

Published In

Aquatic toxicology (Amsterdam, Netherlands)

DOI

EISSN

1879-1514

ISSN

0166-445X

Publication Date

July 2005

Volume

73

Issue

4

Start / End Page

327 / 341

Related Subject Headings

  • Virginia
  • Toxicology
  • Sex Factors
  • Sequence Analysis, DNA
  • RNA, Messenger
  • Oligonucleotide Array Sequence Analysis
  • Molecular Sequence Data
  • Male
  • Liver
  • Geologic Sediments
 

Citation

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ICMJE
MLA
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Meyer, J. N., Volz, D. C., Freedman, J. H., & Di Giulio, R. T. (2005). Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary. Aquatic Toxicology (Amsterdam, Netherlands), 73(4), 327–341. https://doi.org/10.1016/j.aquatox.2005.03.022
Meyer, Joel N., David C. Volz, Jonathan H. Freedman, and Richard T. Di Giulio. “Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary.Aquatic Toxicology (Amsterdam, Netherlands) 73, no. 4 (July 2005): 327–41. https://doi.org/10.1016/j.aquatox.2005.03.022.
Meyer JN, Volz DC, Freedman JH, Di Giulio RT. Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary. Aquatic toxicology (Amsterdam, Netherlands). 2005 Jul;73(4):327–41.
Meyer, Joel N., et al. “Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary.Aquatic Toxicology (Amsterdam, Netherlands), vol. 73, no. 4, July 2005, pp. 327–41. Epmc, doi:10.1016/j.aquatox.2005.03.022.
Meyer JN, Volz DC, Freedman JH, Di Giulio RT. Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a Superfund estuary. Aquatic toxicology (Amsterdam, Netherlands). 2005 Jul;73(4):327–341.
Journal cover image

Published In

Aquatic toxicology (Amsterdam, Netherlands)

DOI

EISSN

1879-1514

ISSN

0166-445X

Publication Date

July 2005

Volume

73

Issue

4

Start / End Page

327 / 341

Related Subject Headings

  • Virginia
  • Toxicology
  • Sex Factors
  • Sequence Analysis, DNA
  • RNA, Messenger
  • Oligonucleotide Array Sequence Analysis
  • Molecular Sequence Data
  • Male
  • Liver
  • Geologic Sediments