Fenoldopam improves corticomedullary oxygen delivery and attenuates angiogenesis gene expression in acute ischemic renal injury.

Published

Journal Article

BACKGROUND/AIMS: Vasoactive compounds are known to affect intrarenal hemodynamics and gene transcription, but specific effects of fenoldopam in the setting of acute renal ischemia are not known. We utilized a rat model of acute ischemic nephropathy to test the hypothesis that fenoldopam improves corticomedullary tissue oxygen tension (PtO2) and attenuates angiogenesis gene expression in acute renal ischemia. METHODS: Rats anesthetized with 50 mg/kg urethane were divided into 4 groups (n = 6 each): (1) sham with infusion of 0.9% saline; (2) sham with infusion of 0.1 microg x kg(-1) x min(-1) fenoldopam; (3) unilateral renal ischemia followed by 6 h of reperfusion with saline, and (4) ischemia/reperfusion with fenoldopam. Renal artery blood flow (RBF), renal cortical perfusion (RCP), and PtO2 were recorded throughout. Total RNA from left kidneys was used to probe microarrays. Gene expression was measured as percent positive control (GAPDH) and confirmed using RT-PCR. RESULTS: Fenoldopam significantly increased RBF (p < 0.05), RCP (p < 0.01) and PtO2 (p <0.01) in both non-ischemic and post-ischemic kidneys. Fenoldopam attenuated 11 of the 13 ischemia-induced genes and 44 of 78 ischemia-suppressed genes. This attenuation was statistically significant (p < 0.05) for five genes. CONCLUSION: Data from this rat model of ischemic nephropathy suggest that fenoldopam improves intrarenal hemodynamics and attenuates ischemia-related changes in angiogenesis gene expression.

Full Text

Cited Authors

  • Aravindan, N; Samuels, J; Riedel, B; Shaw, A

Published Date

  • 2006

Published In

Volume / Issue

  • 29 / 3

Start / End Page

  • 165 - 174

PubMed ID

  • 16931895

Pubmed Central ID

  • 16931895

International Standard Serial Number (ISSN)

  • 1420-4096

Digital Object Identifier (DOI)

  • 10.1159/000095350

Language

  • eng

Conference Location

  • Switzerland