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Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast.

Publication ,  Journal Article
Simmons Kovacs, LA; Nelson, CL; Haase, SB
Published in: Molecular biology of the cell
August 2008

Centrosome duplication must be tightly controlled so that duplication occurs only once each cell cycle. Accumulation of multiple centrosomes can result in the assembly of a multipolar spindle and lead to chromosome mis-segregation and genomic instability. In metazoans, a centrosome-intrinsic mechanism prevents reduplication until centriole disengagement. Mitotic cyclin/cyclin-dependent kinases (CDKs) prevent reduplication of the budding yeast centrosome, called a spindle pole body (SPB), in late S-phase and G2/M, but the mechanism remains unclear. How SPB reduplication is prevented early in the cell cycle is also not understood. Here we show that, similar to metazoans, an SPB-intrinsic mechanism prevents reduplication early in the cell cycle. We also show that mitotic cyclins can inhibit SPB duplication when expressed before satellite assembly in early G1, but not later in G1, after the satellite had assembled. Moreover, electron microscopy revealed that SPBs do not assemble a satellite in cells expressing Clb2 in early G1. Finally, we demonstrate that Clb2 must localize to the cytoplasm in order to inhibit SPB duplication, suggesting the possibility for direct CDK inhibition of satellite components. These two mechanisms, intrinsic and extrinsic control by CDK, evoke two-step system that prevents SPB reduplication throughout the cell cycle.

Duke Scholars

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

August 2008

Volume

19

Issue

8

Start / End Page

3243 / 3253

Related Subject Headings

  • Spindle Apparatus
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Plasmids
  • Models, Biological
  • Mitosis
  • Microscopy, Electron
  • Genome
  • Gene Expression Regulation, Fungal
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Simmons Kovacs, L. A., Nelson, C. L., & Haase, S. B. (2008). Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast. Molecular Biology of the Cell, 19(8), 3243–3253. https://doi.org/10.1091/mbc.e08-02-0148
Simmons Kovacs, Laura A., Christine L. Nelson, and Steven B. Haase. “Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast.Molecular Biology of the Cell 19, no. 8 (August 2008): 3243–53. https://doi.org/10.1091/mbc.e08-02-0148.
Simmons Kovacs LA, Nelson CL, Haase SB. Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast. Molecular biology of the cell. 2008 Aug;19(8):3243–53.
Simmons Kovacs, Laura A., et al. “Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast.Molecular Biology of the Cell, vol. 19, no. 8, Aug. 2008, pp. 3243–53. Epmc, doi:10.1091/mbc.e08-02-0148.
Simmons Kovacs LA, Nelson CL, Haase SB. Intrinsic and cyclin-dependent kinase-dependent control of spindle pole body duplication in budding yeast. Molecular biology of the cell. 2008 Aug;19(8):3243–3253.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

August 2008

Volume

19

Issue

8

Start / End Page

3243 / 3253

Related Subject Headings

  • Spindle Apparatus
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Plasmids
  • Models, Biological
  • Mitosis
  • Microscopy, Electron
  • Genome
  • Gene Expression Regulation, Fungal
  • Developmental Biology