Normal spastin gene dosage is specifically required for axon regeneration.

Published

Journal Article

Axon regeneration allows neurons to repair circuits after trauma; however, most of the molecular players in this process remain to be identified. Given that microtubule rearrangements have been observed in injured neurons, we tested whether microtubule-severing proteins might play a role in axon regeneration. We found that axon regeneration is extremely sensitive to levels of the microtubule-severing protein spastin. Although microtubule behavior in uninjured neurons was not perturbed in animals heterozygous for a spastin null allele, axon regeneration was severely disrupted in this background. Two types of axon regeneration-regeneration of an axon from a dendrite after proximal axotomy and regeneration of an axon from the stump after distal axotomy-were defective in Drosophila with one mutant copy of the spastin gene. Other types of axon and dendrite outgrowth, including regrowth of dendrites after pruning, were normal in heterozygotes. We conclude that regenerative axon growth is uniquely sensitive to spastin gene dosage.

Full Text

Duke Authors

Cited Authors

  • Stone, MC; Rao, K; Gheres, KW; Kim, S; Tao, J; La Rochelle, C; Folker, CT; Sherwood, NT; Rolls, MM

Published Date

  • November 2012

Published In

Volume / Issue

  • 2 / 5

Start / End Page

  • 1340 - 1350

PubMed ID

  • 23122959

Pubmed Central ID

  • 23122959

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

International Standard Serial Number (ISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2012.09.032

Language

  • eng