Reichert's membrane as a model for studying the biosynthesis and assembly of basement membrane components.

Journal Article (Journal Article)

The major components of Reichert's membrane (laminin, type IV procollagen, entactin and heparan sulphate proteoglycan) are all synthesized by the parietal endoderm cells of the mouse embryo. Fibronectin is found mainly on the trophoblast side of Reichert's membrane and does not appear to be a major structural component. Parietal endoderm cells are thought to differentiate and migrate as individual cells from the margins of the epithelial visceral endoderm layer. They may interact with the type IV collagen in Reichert's membrane via a surface-associated protein of Mr = 47000 known as 'colligin'. Parietal endoderm cells are a rich source of mRNAs for basement membrane components and have been used to prepare a cDNA library in the expression vectors pUC8 and pUC9 from which cDNAs for type IV collagen and laminin B chains have been isolated.

Full Text

Duke Authors

Cited Authors

  • Hogan, BL; Barlow, DP; Kurkinen, M

Published Date

  • 1984

Published In

Volume / Issue

  • 108 /

Start / End Page

  • 60 - 74

PubMed ID

  • 6569831

International Standard Serial Number (ISSN)

  • 0300-5208

Digital Object Identifier (DOI)

  • 10.1002/9780470720899.ch5


  • eng

Conference Location

  • Netherlands